The contemporary study reveals the potential of dead immobilized Rhizopus Arrhizus (DIRA) by studying the effects of initial pH of solution, contact time, initial Cu(II) concentration, co-ion, desorption and biosorbent reuse on the uptake of Cu(II) from synthetic solution. Optimum pH for Cu(II) biosorption on DIRA was 6.5. Pseudo secondorder kinetic most suitably elucidated the kinetic trend. Removal of Cu(II) by DIRA followed the Freundlich adsorption isotherm. Experiments on effect of temperature revealed endothermic nature of Cu(II) biosorption upon DIRA. Na+ and Ca++ showed synergetic effect, while Mg++ and Zn++ revealed antagonistic effect on the biosorption of Cu(II). It was shown that reuse of DIRA was possible by means of 0.1 M HCl as an eluent.