Porcine amniotic fluid was investigated for use as a functional cosmetic ingredient. From safety tests by MTT (5-diphenyltetrazolium bromide) assay, cell viability was above 90% for 50-1,000 μg/mL concentration and porcine amniotic fluid was safe for cosmetic ingredient. From stability tests, cream containing 1% porcine amniotic fluid maintained constant physical properties for color, pH and viscosity during 28 days, and porcine amniotic fluid was stable for a cosmetic agent. Efficacy tests were done for antiwrinkle (elastase inhibition and collagenase synthesis inhibition), whitening (tyrosinase inhibition and DOPA (3,4-Dihydroxy-L-phenyl-alanine) oxidation inhibition) and antioxidation. At 500 μg/mL concentration, elastase inhibition of porcine placenta amniotic fluid was 33%, whereas that of adenosine as reference was 14%. However, porcine amniotic fluid showed relatively insignificant effect on collagenase synthesis inhibition, whitening and antioxidation activity. From this study, porcine amniotic fluid showed potential for a future antiwrinkle cosmetic agent.