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Issue
Korean Journal of Chemical Engineering,
Vol.28, No.5, 1260-1265, 2011
Inhibitory activity of Phellodendri cortex extracts on differentiation of 3T3-L1 preadipocytes
Choi DK, Oh TS, Yun JW
The purpose of the present study was to investigate the inhibitory effect of beberine-rich fraction of Phellodendri cortex extract (PC) on adipogenesis in 3T3-L1 cells. PC effectively prevented TG accumulation in differentiation of 3T3-L1 preadipocytes in a dose-dependent manner. Compared to controls, PC at a concentration of 75 μg/mL significantly decreased lipid droplets by 79.5%. Beberine exhibited similar inhibitory effect to that of PC extract, suggesting that fractionated PC extract contained mostly berberine. The expression levels of PAARγ and C/EBPα were significantly reduced by PC treatment. This result consequently led to suppression of expression of LPL, FABP4, CD36, and SCD2, which are target genes of PPARγ and C/EBPα. PC also inhibited protein levels of PPARγ, FABP4, and GLUT4. Accordingly, these results suggest that PC inhibited adipogenesis in 3T3-L1 preadipocyte cells through PPARγ and CEBPα-related pathways at both transcript and protein levels.
Keywords: Phellodendri cortex; Berberine; C/EBPα; PPARγ; 3T3-L1 Preadipoctye
[References]
  1. Ambati S, Yang JY, Rayalam S, Park HJ, Della-Fera MA, Baile CA, Phytother Res., 23, 513, 2009
  2. Berger JP, Akiyama TE, Meinke PT, Trends Pharmacol Sci., 26, 244, 2005
  3. Berggren JR, Hulver MW, Houmard JA, J. Appl. Physiol., 99, 757, 2005
  4. Chinetti-Gbaguidi G, Fruchart JC, Staels B, Curr. Opin. Pharmacol., 5, 177, 2005
  5. Chinetti-Gbaguidi G, Fruchart JC, Staels B, Biomarkers., 10, S30, 2005
  6. Choi BH, Ahn IS, Kim YH, Park JW, Lee SY, Hyun CK, Do MS, Exp. Mol. Med., 38, 599, 2006
  7. Choi SS, Cha BY, Lee YS, Yonezawa T, Teruya T, Nagai K, Woo JT, Life Sci., 84, 908, 2009
  8. Cornelius P, MacDougald OA, Lane MD, Annu. Rev. Nutr., 14, 99, 1994
  9. Ejaz A, Wu D, Kwan P, Meydani M, J. Nutr., 139, 919, 2009
  10. Evans RM, Barish GD, Wang YX, Nat. Med., 10, 355, 2004
  11. Green H, Meuth M, Cell., 3, 127, 1974
  12. Gregoire FM, Smas CM, Sul HS, Phys. Rev., 78, 783, 1998
  13. Hasegawa N, Phytother. Res., 15, 172, 2001
  14. Hu Y, Davies GE, Phytomedicine., 16, 864, 2009
  15. Janke J, Engeli S, Gorzelniak K, Luft FC, Sharma AM, Obes. Res., 10, 1, 2002
  16. Jeon T, Hwang SG, Hirai S, Matsui T, Yano H, Kawada T, Lim BO, Park DK, Life Sci., 75, 3195, 2004
  17. Kahn BB, Cell., 92, 593, 1998
  18. Kawada T, Takahashi N, Fushiki T, J. Nutr. Sci. Vitaminol., 47, 1, 2001
  19. Kim HJ, Kong MK, Kim YC, BMB Rep., 41, 710, 2008
  20. Lee YS, Kim WS, Kim KH, Yoon MJ, Cho HJ, Shen Y, Ye JM, Lee CH, Oh WK, Kim CT, Hohnen-Behrens C, Gosby A, Kraegen EW, James DE, Kim JB, Diabetes., 55, 2256, 2006
  21. Liu W, Liu P, Tao S, Deng Y, Li X, Lan T, Zhang X, Guo F, Huang W, Chen F, Huang H, Zhou SF, Arch. Biochem. Biophys., 475, 128, 2008
  22. Liu W, Tang F, Deng Y, Li X, Lan T, Zhang X, Huang H, Liu P, Mol. Cell Biochem., 325, 99, 2009
  23. Maeda H, Hosokawa M, Sashima T, Takahashi N, Kawada T, Miyashita K, Int. J. Mol. Med., 18, 147, 2006
  24. Maeda K, Cao H, Kono K, Gorgun CZ, Furuhashi M, Uysal KT, Cao Q, Atsumi G, Malone H, Krishnan B, Minokoshi Y, Kahn BB, Parker RA, Hotamisligil GS, Cell Metab., 1, 107, 2005
  25. Mendes V, Monteiro R, Pestana D, Teixeira D, Calhau C, Azevedo I, J. Agric. Food Chem., 56, 11631, 2008
  26. Moller DE, Nature., 414, 821, 2001
  27. Mori H, Fuchigami M, Inoue N, Nagai H, Koda A, Nishioka I, Planta Med., 60, 445, 1994
  28. Mori H, Fuchigami M, Inoue N, Nagai H, Koda A, Nishioka I, Meguro K, Planta Med., 61, 45, 1995
  29. Pakala R, Kuchulakanti P, Rha SW, Cheneau E, Baffour R, Waksman R, Cardiovasc. Radiat. Med., 5, 97, 2004
  30. Park JI, Shim JK, Do JW, Kim SY, Seo EK, Kwon HJ, Lee TK, Kim JK, Choi DY, Kim CH, Glycoconj J., 16, 247, 1999
  31. Sharma AM, Int. J. Obes. Relat. Metab. Disord., 26, S5, 2002
  32. Shimada T, Hiramatsu N, Kasai A, Mukai M, Okamura M, Yao J, Huang T, Tamai M, Takahashi S, Nakamura T, Kitamura M, Am. J. Phys. Endocrinol. Metab., 295, E859, 2008
  33. Staels B, Fruchart JC, Diabetes., 54, 2460, 2005
  34. Wu Z, Xie Y, Bucher NL, Farmer SR, Genes Dev., 9, 2350, 1995
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