| Issue |
Korean Journal of Chemical Engineering,
Vol.27, No.2, 587-589, 2010
Vol.27, No.2, 587-589, 2010
Overexpression of ethionine resistance gene for maximized production of S-adenosylmethionine in Saccharomyces cerevisiae sake kyokai No. 6
In our previous work, we developed an auxotrophic mutant of Saccharomyces cerevisiae sake kyokai No. 6 strain for the selection of recombinant DNA. Sake yeast can accumulate high amount of S-adenosylmethionine (SAM) that is effective for treating depression, Alzheimer’s disease and so on. In this work, we further introduced SAM2 gene and ERC1, a frame-shift mutant of YHR032w, to maximize the production of S-adenosylmethionine in S. cerevisiae sake K6. The recombinant strain accumulated SAM about half of its dry cell weight.
[References]
- Cantoni GL, Annu. Rev. Biochem., 44, 435, 1975
- Lu SC, Int. J. Biochem. Cell. Biol., 32, 391, 2000
- Mato JM, Corrales FJ, Lu SC, Avila MA, Faseb J., 16, 15, 2002
- Mischoulon D, Fava M, Am. J. Clin. Nutr., 76, 1158S, 2002
- Fuso A, Seminara L, Cavallaro RA, D’Anselmi F, Scarpa S, Mol. Cell. Neurosci., 28, 195, 2005
- Roje S, Wang H, McNeil SD, Raymond RK, Appling DR, Shachar-Hill Y, Bohnert HJ, Hanson, AD, J. Biol. Chem., 274, 36089, 1999
- Choi ES, Park BS, Lee SW, Oh MK, Korean J. Chem. Eng., 26(1), 156, 2009
- Shiomi N, Fukuda H, Fukuda Y, Murata K, Kimura A, J. Ferment. Bioeng., 71, 211, 1991
- Shobayashi M, Ukena E, Fujii T, Iefuji H, Biosci. Biotechnol. Biochem., 71, 323, 2007
- Budovskaya YV, Stephan JS, Deminoff SJ, Herman PK, Proc. Natl. Acad. Sci. USA, 102, 13933, 2005
- Sreenivas A, Carman GM, J. Biol. Chem., 278, 20673, 2003
- Higuchi R, Krummel B, Saiki RK, Nucleic Acids Res., 16, 7351, 1988
- Schwencke J, De Robichon-Szulmajster H, Eur. J. Biochem., 65, 49, 1976
- Roje S, Wang H, McNeil SD, Raymond RK, Appling DR, Shachar-Hill Y, Bohnert HJ, Hanson AD, J. Biol. Chem., 274, 36089, 1999
참고문헌정보(참고문헌, 인용문헌)는 화학공학소재연구정보센터에 의해 제공되고 있습니다.