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Issue
Korean Journal of Chemical Engineering,
Vol.27, No.1, 183-186, 2010
Strain improvement of Rhizopus oryzae for over-production of fumaric acid by reducing ethanol synthesis pathway
Fu YQ, Xu Q, Li S, Chen Y, Huang H
Rhizopus oryzae mutants were isolated from the wild type strain ME-F01 after mutagenesis with UV coupled with nitrosoguanidine (NTG) and the following allyl alcohol resistance selection method. By analyzing the activities of alcohol dehydrogenase (ADH) involved in ethanol synthesis pathway and batch fermentation, one mutant, ME-UN-8, was isolated that produced 21.1% more fumaric acid than ME-F01 with the corresponding byproduct of ethanol decreased by 83.7%.
Keywords: Alcohol Dehydrogenase; Allyl Alcohol; Fumaric Acid; Mutation; Rhizopus oryzae
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