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Issue
Korean Journal of Chemical Engineering,
Vol.26, No.1, 258-260, 2009
Applications of silver nanoplates as colorimetric indicators of pH-induced conformational changes in cytochrome c
Park JS, Yoon DY, Kim YH
Silver nanoplates (AgNPs) were used as a colorimetric indicator of pH-induced conformational changes in the protein, cytochorme c (cyt c). Cyt c was covalently bound to the surface of AgNPs, and UV-vis spectroscopy was used to identify changes in the optical properties of the resulting cyt c-AgNP conjugates in colloidal solution over pH, with values ranging from 3.1 to 10.6. Transmission electron microscopy (TEM) results indicated that the morphological changes in cyt c-AgNP conjugates were dependent on pH. Moreover, pH-induced conformational changes in cyt c could be detected by visual inspection of the color changes in cyt c-AgNPs, which occurred as a result of the coupling effect of localized surface plasmon resonance (LSPR) by aggregated AgNPs.
Keywords: Silver Nanoparticle; Colorimetric; Cytochrome; pH
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