Print: | ISSN 0256-1115 |
Online: | ISSN 1975-7220 |
Korean Journal of Chemical Engineering,
Vol.26, No.1, 136-140, 2009 Properties of bacterial cellulose produced in a pilot-scale spherical type bubble column bioreactor
The saccharogenic liquid obtained by the enzymatic saccharification of food wastes was used as a medium for production of bacterial cellulose (BC). The enzymatic saccharification of food wastes (SFW) was carried out by the cultivation supernatant of Trichoderma inhamatum KSJ1. 5.6 g/L of BC was produced in a new modified 50 L bubble column bioreactor by Acetobacter xylinum KJ1. The productivity was similar to that of a modified 10 L bubble column bioreactor (5.8 g/L). When pure oxygen was supplied into the scaled-up culture conditions, 6.8 g/L (12% enhancement) of BC was produced, indicating a very useful method for BC mass production. The oxygen uptake rate (OUR) and qO2 (specific oxygen uptake rate) were 0.214 mg-DO/L·min and 0.257 mg-DO/g-cell·min, respectively. The physical properties, such as morphology, molecular weight, crystallinity, and tensile strength of BCs produced in static culture (A), 10 L (B) and 50 L (C) modified bubble column cultures were investigated. All BCs showed fibrils
with highly networking structure. The number average molecular weight of BCs in A, B and C was 2,314,000, 1,878,000, and 1,765,000, respectively. All of the BCs had a form of cellulose I representing pure cellulose. The relative degree of crystallinity showed the range of 79.6-86.0%. Tensile strengths of BC sheet in A, B and C were 1.75, 1.21, and 1.19 kg/mm^(2), respectively. In conclusion, BC production by the modified bubble column culture mode of 50 L brought more
favorable results in terms of the physical properties and its ease of scale-up.
Keywords:
Saccharification of Food Wastes (SFW); Bacterial Cellulose (BC); Pilot Scale Production; Modified Bubble Column Bioreactor; Physical Properties
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