Korean Journal of Chemical Engineering, Vol.24, No.5, 806-811, 2007
Purification and characterization of a thermophilic chitinase produced by Aeromonas sp. DYU-Too7
An extracellular chitinase, produced by Aeromonas sp. DYU-Too7, was purified in the following procedures: ammonium sulfate precipitation, ultrafiltration, chromatographic separation of DEAE-sepharose CL-6B and sephacryl S-100HR. The resulting chitinase has a molecular mass of 36 kDa, an optimal reaction pH of 5.0, and an optimal reaction temperature of 70 ℃. It retains almost 100% activity in the pH range of 5.0-8.0. This chitinase has a high thermal tolerance and retained 90% of its activity at 50 ℃ and 75% at 60 ℃. Enzyme activity was inhibited by Ba2+, Hg2+, Mg2+ and Ag+ cations, but was not substantially inhibited by the K+ cation nor the chelating agent EDTA. The Km and Vmax, using colloidal chitin as a substrate, are 6.3 g/L and 18.69 μmol/min/mg-protein, respectively. The 36 kDa chitinase of Aeromonas sp. DYU-Too7 is an exo-type enzyme, because chitobiose was the main hydrolysate in hydrolysis of colloidal chitin.
[References]
Flach J, Pilet PE, Jolles P, Experientia. , 48 , 701, 1992
Zheng LY, Xiao YL, Korean J. Chem. Eng. , 21 (1), 201, 2004
Tsai GJ, Wu ZY, Su WH, J. Food Prot. , 63 , 747, 2000
Tokoro A, Kobayashi M, Tatewaki N, Suzuki K, Okawa Y, Mikami T, Suzuki S, Suzuki M, Microbiol. Immunol. , 33 , 357, 1989
Tokoro A, Tatewaki N, Suzuki K, Mikami T, Suzuki S, Suzuki M, Chem. Pharm. Bull. , 36 , 784, 1988
Chang KB, Lee J, Fu WR, J. Food Drug Anal. , 8 , 75, 2000
Sakurad M, Morgavi DP, Komatani K, Tomita Y, Onodera R, Curr. Microbiol. , 35 , 48, 1997
Kupiec CR, Chet I, Curr. Opin. Biotechnol. , 9 , 270, 1998
Gooday GW, Biodegradation , 1 , 177, 1990
Harman GE, Hayes CK, Lorito M, Broadway RM, Dipietro A, Peterbauer C, Tronsmo A, Phytopathology , 83 , 313, 1993
Sitrit Y, Vorgias CE, Chet I, Oppenheim AB, J. Bacteriol. , 177 , 4178, 1995
Ueda M, Kojima M, Yoshikawa T, Mitsuda N, Araki K, Kawaguchi T, Miyatake K, Arail M, Fukamizo T, Eur. J. Biochem. , 270 , 2513, 2003
Lien TS, Too JR, Wu ST, Yu ST, J. Food Biochem. , 29 , 422, 2005
Pitpreecha S, Damrongsakkul S, Korean J. Chem. Eng. , 23 (6), 972, 2006
Imoto I, Yagishita K, Agric. Biol. Chem. , 72 , 1154, 1971
Hsu SC, Lockwood JL, Appl. Microbiol. , 29 , 422, 1975
Wiwat C, Siwayapahm P, Bhumiratana A, Curr. Microbiol. , 39 , 134, 1999
Bradford MM, Anal. Biochem. , 72 , 248, 1976
Laemmli UK, Nature , 227 , 680, 1970
Trudel J, Asselin A, Anal. Biochem. , 178 , 362, 1989
Guo SH, Chen JK, Lee WC, Enzyme Microb. Technol. , 35 (6-7), 550, 2004
Lan X, Ozawa N, Nishiwaki N, Kodaira R, Okazaki M, Shimosaka M, Biosci. Biotechnol. Biochem. , 68 , 1082, 2004
Lin CS, Chen HC, Lin FP, Enzyme Microb. Technol. , 21 (7), 472, 1997
Hiraga K, Shou L, Kitazawa M, Takahashi S, Shimada M, Sato R, Oda K, Biosci. Biotechnol. Biochem. , 61 , 174, 1997
Sutrisno A, Ueda M, Inui H, Kawaguchi T, Nakano Y, Arai M, Miyatake K, J. Biosci. Bioeng. , 91 , 599, 2001
Nawani NN, Kapadnis BP, Das AD, Rao AS, Mahajan SK, J. Appl. Microbiol. , 93 , 965, 2002
Ueda M, Fujiwara A, Kawaguchi T, Arai M, Biosci. Biotechnol. Biochem. , 59 , 2162, 1995
Thompson SE, Smith M, Wilkinson MC, Peek K, Appl. Environ. Microbiol. , 67 , 4001, 2001
Ohishi K, Yamagishi M, Ohta T, Suzuki M, Izumida H, Sano H, Nishijima M, Miwa T, J. Ferment. Technol. , 82 , 598, 1996
Xia G, Jin C, Zhou J, Yang S, Zhang S, Jin C, Eur. J. Biochem. , 268 , 4079, 2001
Gupta R, Saxena RK, Chaturvedi P, Virdi SS, J. Appl. Microbiol. , 78 , 378, 1995
Ueda M, Arai M, Biosci. Biotechnol. Biochem. , 56 , 1204, 1992
Wang SY, Moyne AL, Thottappilly G, Wu SJ, Locy RD, Singh NK, Enzyme Microb. Technol. , 28 (6), 492, 2001
Arora N, Ahmad T, Rajagopal R, Bhatnagar RK, Biochem. Biophys. Res. Commun. , 307 , 620, 2003
Wen CM, Tseng CS, Cheng CY, Li YK, Biotechnol. Appl. Biochem. , 35 , 213, 2002
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