A recycling preparative HPLC was used to extract and separate the eupatilin contained in Artemisia princepsPAMPAN, and the optimum operating condition was experimentally determined. Eupatilin was extracted by ethanolsolvent from the leaf and trunk of Artemisia princeps PAMPAN. The resulting solution was further partitioned withn-hexane, chloroform and ethyl acetate. The solution containing eupatilin was collected using a preparative as wellas analytical column, and it was identified by LC-CE-MS. In the experiment, the mobile phase consisted of water/aceto-nitrile/TFA=50/50/0.5 (vol%), and UV wavelength measured was 370nm. For analytical chromatography, the injectionvolume was 20μL and the flow rate was fixed at 1.0mL/min. The measured retention time for eupatilin was approxi-mately 8.6min in the above operating condition. For recycling preparative HPLC with commercially available GSA310A column at 1.5mL/min of the flow rate and 2mL of injection volume, the purity of the eupatilin was almost 100%after recycling twice.