A few sections of the sewers in Watertown were experiencing clogging due to an excess biomass growth. It was suspected that the wastewaters discharged from Fisher-Barton’s two facilities were causing excess biomass growth. Madison raw sewage had a slightly lower biomass growth than the mixture of industrial wastewater and domestic sewage samples. From microscopic examination of biomass, the main cause of excess biomass growth is thought to be toilet tissue. Biomass is attached to toilet tissue, decomposing it gradually and generating extracellular polymeric substances (EPS). Then, suspended particulates including graphite and precipitates are attached to the biomass and filamentous organisms, especially Sphaerotilus natans, leading to the increase in the biomass volume. We found that the filamentous bacteria were present in biofilm by FISH. A significant amount of graphite was embedded to biomass grown in sewer. Since the major cause of excess biomass appeared to be toilet tissue, it would not be economical to employ a filtration system for complete graphite separation. Instead, it is recommended that the manufacturing process where graphite is used be assessed and a best management practice is in place.
Borsodi AK, Farkas I, Kurdi P, Water Res., 32, 1831, 1998
Bott TR, "Biofilms in Process and Industrial Waters," In; Biofilms in the Aquatic Environment (eds.; Keevil, C.W., Godfree, A., Holt, D., Dow, C.) Cambridge, The Royal Society of Chemistry (RSC), 80, 1999
Cao YS, Alaerts GJ, Water Sci. Technol., 37, 181, 1995
Eikelboom DH, Water Res., 9, 365, 1973
Flemming HC, Wingender J, Moritz R, Borchard W, Mayer C, "Physico-Chemical Properties of Biofilms," A. Short Review, In; Biofilms in the Aquatic Environment (eds.; Keevil, C.W., Godfree, A., Holt, D., Dow, C.) Cambridge, The Royal Society of Chemistry (RSC), 1, 1999
Jahn A, Nielsen PK, Water Sci. Technol., 37, 17, 1998
Manz W, Wagner M, Amann R, Schleifer KH, Water Res., 28, 1715, 1994
Manz W, Wendt-Potthoff K, Neu TR, Szewzyk U, Lawrence JR, Micro. Ecol., 37, 225, 1999
MacDonald JA, "Microscopic Evaluation of a Collection System Sample Received from Watertown," Wisconsin, Final Report, MacDonald Environmental Services, Project No. 00-31, 2000
McFeters GA, Stewart PS, Huang CT, Wentland EJ, Xu KD, Yu FP, "Physiological Heterogeneity and Biofilm Control," In; Biofilms in the Aquatic Environment (eds.; Keevil, C.W., Godfree, A., Holt, D., Dow, C.) Cambridge, The Royal Society of Chemistry (RSC), 51, 1999
Metcalf & Eddy, "Wastewater Engineering: Collection and Pumping of Wastewater," 3rd ed., McGraw-Hill, New York, NY, 1991
Nielsen PH, Andreasen K, Wagner M, Blackall LL, Lemmer H, Seviour RJ, Water Sci. Technol., 37, 423, 1998