| Issue |
Korean Journal of Chemical Engineering,
Vol.19, No.5, 815-817, 2002
Vol.19, No.5, 815-817, 2002
Chromatographic Analysis of Ceramide III of Saccharomyces cerevisiae
Ceramide was prepared by the cultivation of Saccharomyces cerevisiae from cell extracts by solvent extraction and analyzed by NP-HPLC using a UV detector. The mobile phase was composed of hexane, methanol, and IPA. From the experimental conditions, the composition of mobile phase was 72/5/23 (hexane/IPA/methanol, vol%). Quantitative analysis of ceramide was performed. Based on the analytical conditions, the effect of cultivation temperature for the production of ceramide was investigated and the optimum cultivation temperature was found to be 35 ℃.
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