Isoprene is an important volatile organic compound causing photochemical smog in the atmosphere; thus, accurate analysis of isoprene is essential. In this study, the effect of sampling conditions, including adsorbent types, sampling temperatures, and flow rates on the recovery of isoprene, was investigated. Common adsorption traps of isoprene, including Tenax TA/Carbosieve SIII, Tenax TA/Carbotrap, were used as adsorbents. Sampling temperatures varied from 25 oC to 40 oC. Sampling flow rates were 50, 100, and 200 mL min-1. It was found that the Tenax/Carbotrap trap revealed the highest isoprene recovery rate; however, the Tenax/Carbosieve SIII trap depicted more significant loss of isoprene than the other one. As for sampling variables, the lower the temperatures and flow rates concerned were, the higher the isoprene recovery was. It was concluded that sampling temperatures and flow rates should be ≤35 oC and ≤50 mL min-1 during a sampling process, respectively. In addition, Carbosieve SIII should not be used for isoprene sampling due to its poor recovery rate.