Sea buckthorn (SBT) combines very fascinating nutritional composition with vital vitamins (A, C, E, D, K, and B complexes). Flavonoids, sterols, α-carotene, linoleic acid, and many more unsaturated fatty acids are present in the sea buckthorn plant. The organic extract of SBT seeds is commonly utilized as an anti-aging ingredient in numerous cosmetics. SBT oil extracts are used in pharmaceuticals that treat diseases like diabetes, cancer, cardiovascular disease, and neurological disorders, in addition to cosmetology. In this investigation, various concentrations of organic solvents such as n-hexane, isopropyl alcohol, ethyl acetate, ethanol, methanol, and ascorbic acid (standard) were used for the extraction of oil from sea buckthorn seeds. The antioxidant activity of such extracts was checked by the iron chelating, commonly known as the ferric chloride (FeCl3) method, which is based on the ferric reducing ability of plasma (FRAP) assay with the help of UV-Vis. Our results indicate that seed extract of Hippophae rhamnoides, should be considered as a non-toxic source and the ferric reducing ability of plasma (FRAP) assay is used to evaluate the anti-oxidant potential by various organic solvents. The highest (68%) of FRAP is scavenged by the ethyl acetate and least (53%) of isopropyl extracts.