About The Journal
  Aims and Scope
  Editorial Board
  Submit a Manuscript 
  Subscription Information
  Guidelines for Publication
  Ethics
Articles & Issues
  Search
  Issue
  Online First
  KJChE on
For Authors
  Instructions to Authors
  Ethical Responsibilities of
  Authors in KJChE
  Ethics in Publishing
  Peer Review Process
  Author's Checklist
  Copyright Transfer Form
Contact us
 
 
 
Issue
Korean Journal of Chemical Engineering,
Vol.40, No.2, 369-378, 2023
Development of a liquid-based cytology method for detecting cervical cancer cells using functional gold nanorods
Jeong ES, Park JJ, Kim HY, Lee SJ, Choi YH, Tanaka M, Choi JH
A cytopathology test for cervical cancer diagnosis has been introduced to complement the histopathology test. This study developed a new cervical cancer diagnostic method that complements the existing liquid cytology to reduce the diagnostician’s skill dependence and to improve diagnostic accuracy. With the ease of introduction of surface functional groups and their high biocompatibility and stability, gold nanomaterials have been used to diagnose numerous biomolecules. CA125 and p16 markers were chosen as protein markers uniquely present in cervical cancer. Bioconjugation technology was used to form a stable bond between the target antibody and gold nanorods. Antibodygold nanorods were administered to cervical cancer cells as a factor for optical microscopic analysis. The degree of cancer cell labeling was determined through additional fluorescence microscopy analysis. Additionally, a liquid-based cytology test mimicking the clinical environment was prepared, and the false-positive rate, false-negative rate, and accuracy were determined when the study strategy was implemented. The strategy validated in this study (antibody-gold nanorodbased cervical cancer diagnosis) suggests a method that can reduce the dependence on experts for the cancer cytology and can increase test accuracy.
Keywords: Cervical Cancer; Cytology; Gold Nanorod; CA125; p16; Cancer Diagnosis
[References]
  1. Waggoner SE, Lancet, 361, 9376, 2003
  2. Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A, Cancer J. Clinicians, 68, 6, 2018
  3. Cohen PA, Jhingran A, Oaknin A, Denny L, Lancet, 393, 10167, 2019
  4. Duenas-Gonzalez A, Lizano M, Candelaria M, Cetina L, Arce C, Cervera E, Mol. Cancer, 4, 1, 2005
  5. Kessler TA, Semin Oncol Nurs., 33, 2, 2017
  6. Dasari S, Wudayagiri R, Valluru L, Clin. Chim. Acta, 445, 7, 2015
  7. Madrid-Marina V, Torres-Poveda K, Lopez-Toledo G, Garcia-Carranca A, Arch. Med. Res., 40, 6, 2009
  8. Harper DM, Franco EL, Wheeler C, Ferris DG, Jenkins D, Schuind A, Zahaf T, Innis B, Naud P, De Carvalho NS, Lancet, 364, 9447, 2004
  9. Koutsky LA, Ault KA, Wheeler CM, Brown DR, Barr E, Alvarez FB, Chiacchierini LM, Jansen KU, N. Engl. J. Med., 347, 1645, 2002
  10. Wentzensen N, Sherman ME, Schiffman M, Wang SS, Gynecol Oncol., 112, 293, 2009
  11. Shingleton HM, Patrick RL, Johnston WW, Smith RA, CA Cancer J. Clin., 45, 5, 1995
  12. Papanicolaou GN, Traut HF, Arch. Pathol. Lab. Med., 121, 3, 1997
  13. Khan MS, Raja FY, Ishfaq G, Tahir F, Subhan F, Kazi BM, Karamat KA, Pak. J. Med. Res., 44, 111, 2005
  14. Cronje HS, Int. J. Gynaecol Obstet., 84, 101, 2004
  15. Gibb RK, Martens MG, Rev. Obstet. Gynecol., 4, S2, 2011
  16. Gabriel C, Achten R, Drijkoningen M, Acta Cytol., 48, 6, 2004
  17. Singh U, Qureshi S, Negi N, Singh N, Goel M, Srivastava K, Ind. J. Med. Res., 147, 3, 2018
  18. Jin J, Yue CY, J. Lab. Med., 44, 3, 2020
  19. de Bekker-Grob EW, de Kok IM, Bulten J, van Rosmalen J, Vedder JE, Arbyn M, Klinkhamer PJ, Siebers AG, van Ballegooijen M, Cancer Causes Control, 23, 8, 2012
  20. Dykman L, Khlebtsov N, Chem. Soc. Rev., 41, 6, 2012
  21. Dreaden EC, Alkilany AM, Huang X, Murphy CJ, El-Sayed MA, Chem. Soc. Rev., 41, 7, 2012
  22. Boisselier E, Astruc D, Chem. Soc. Rev., 38, 6, 2009
  23. Newhouse RJ, Zhang JZ, Reviews in Plasmonics 2010, Springer Nature, Switzerland (2012).
  24. Mout R, Moyano DF, Rana S, Rotello VM, Chem. Soc. Rev., 41, 7, 2012
  25. Dykman LA, Khlebtsov NG, Chem. Rev., 114, 2, 2014
  26. Burgi T, Nanoscale, 7, 38, 2015
  27. Jazayeri MH, Amani H, Pourfatollah AA, Pazoki-Toroudi H, Sedighimoghaddam B, Sensing Bio-sensing Research, 9, 17, 2016
  28. Goldberg GL, Sklar A, O'Hanlan KA, Levine PA, Runowicz CD, Gynecol Oncol., 40, 3, 1991
  29. Shen H, Guo M, Wang L, Cui X, Genes Genomics, 42, 2, 2020
  30. Bender DP, Sorosky JI, Buller RE, Sood AK, Am. J. Obstet. Gynecol., 189, 1, 2003
  31. Porika M, Vemunoori AK, Tippani R, Mohammad A, Bollam SR, Abbagani S, Asian Pac. J. Cancer Prev., 11, 6, 2010
  32. Zhou LL, Shen Y, Gong JM, Sun P, Sheng JH, Oncotarget, 8, 41, 2017
  33. Crawford A, Haber L, Kelly MP, Vazzana K, Canova L, Ram P, Pawashe A, Finney J, Jalal S, Chiu D, Colleton CA, Garnova E, Makonnen S, Sci. Transl. Med., 11, 497, 2019
  34. Roelens J, Reuschenbach M, von Knebel Doeberitz M, Wentzensen N, Bergeron C, Arbyn M, Cancer Cytopathol., 120, 5, 2012
  35. Romagosa C, Simonetti S, Lopez-Vicente L, Mazo A, Lleonart ME, Castellvi J, y Cajal SR, Oncogene, 30, 18, 2011
  36. Munger K, Werness BA, Dyson N, Phelps WC, Harlow E, Howley PM, Embo J., 8, 13, 1989
  37. Reuschenbach M, Waterboer T, Wallin KL, Einenkel J, Dillner J, Hamsikova E, Eschenbach D, Zimmer H, Heilig B, Int. J. Cancer, 123, 11, 2008
  38. Haller F, Agaimy A, Cameron S, Beyer M, Gunawan B, Happel N, Langer C, Ramadori G, von Heydebreck A, Fuzesi L, Histopathology, 56, 3, 2010
  39. Bastide K, Guilly MN, Bernaudin JF, Joubert C, Lectard B, Levalois C, Malfoy B, Chevillard S, Lung Cancer, 63, 3, 2009
  40. Schwabe M, Lubbert M, Curr. Pharm. Biotechnol., 8, 6, 2007
  41. Ye X, Zheng C, Chen J, Gao Y, Murray CB, Nano Lett., 13, 2, 2013
  42. Monsonego J, Hudgens MG, Zerat L, Zerat JC, Syrjanen K, Halfon P, Ruiz F, Smith JS, Int. J. Cancer, 129, 3, 2011
  43. Kirschner B, Simonsen K, Junge J, Cytopathology, 17, 4, 2006
  44. Kaufman HW, Alagia DP, Chen Z, Onisko A, Austin RM, Am. J. Clin. Pathol., 154, 4, 2020
  45. Tanabodee J, Thepsuwan K, Karalak A, Laoaree O, Krachang A, Manmatt K, Anontwatanawong N, Asian Pac. J. Cancer Prev., 16, 16, 2015
  46. Ikenberg H, Bergeron C, Schmidt D, Griesser H, Alameda F, Angeloni C, Bogers J, Dachez R, Denton K, Hariri J, Keller T, J. Natl. Cancer Inst., 105, 20, 2013
  47. Mishra GA, Pimple SA, Shastri SS, Ind. J. Med. Paediatr. Oncol., 32, 3, 2011
참고문헌정보(참고문헌, 인용문헌)는 화학공학소재연구정보센터에 의해 제공되고 있습니다.