An ultrasound-negative pressure cavitation extraction method was developed to remarkably improve the recovery efficiency of paclitaxel from Taxus chinensis. The paclitaxel yield was 94-100% through ultrasound-negative pressure cavitation extraction with an extraction time of 3 to 8min. In particular, most paclitaxel could be recovered within 3min of extraction at ultrasonic power of 380W/negative pressure of -260mmHg. Observation of the biomass surface with SEM before and after extraction showed that as the ultrasonic power and negative pressure increased, the surface was more disrupted. In addition, a pseudo-second order model was suitable for the kinetic analysis, and intraparticle diffusion played a dominant role in the overall extraction rate according to the intraparticle diffusion model. As the ultrasonic power and negative pressure increased, the extraction rate constant (6.8816-11.6105mL/mg·min), the effective diffusion coefficient (1.550 x 10-12-11.528 x 10-12m2/s), and the mass transfer coefficient (2.222 x 10-7-5.149 x 10-7 m/s) increased.