Passive direct methanol fuel cells (DMFC) are applicable for charging portable electronic devices. In passive DMFC, fuel and oxidants are supplied through diffusion and natural convection process. The present experimental work analyzed the effect of the membrane electrode assembly (MEA) activation, methanol concentration, bolt tightening torque and stability of the fuel cell stack. Newly fabricated MEA were activated for different time durations of 0, 6, 12 and 18 hrs at 1M of methanol concentration with a constant load. The concentration of methanol varied from 1M to 6M and also bolt torque varied from 4N-m to 8N-m. Further, open circuit voltage (OCV) and voltage stability with respect to time were analyzed. From the results, it is observed that the fuel cell performance was enhanced from 1M to 5M and then decreased. From 0-12 hrs, the cell performance increased with respect to time and then continued the same performance at the 18th hr. From the results, it is also observed that increased bolt torque from 4N-m to 7N-m enhanced the fuel cell performance and then decreased. The fuel cell performance was analyzed in terms of maximum power density and maximum current density.