Two samples of multi-layered depth filter media and single layers were prepared for this study. Filtration performance of these samples was evaluated using lab scale test unit with KCl as test aerosol. Filter media samples were composed of three layers, two of them of meltblown fiber layers and one of thermal-bonded microfiber layer. A commercial filter media was used as reference sample to compare the filtration performance. Quality factors were calculated in addition to evaluate the overall filtration performance of the new composite filter media. The results indicate a satisfactory filtration efficiency of the media M2U, over 90% for the studied particle size range. Compared to reference media, new media M2U shows better performance, especially for the particle size greater than 50 nm. Charged (M2U) and uncharged (M2U-2) composite media were tested and results compared with theory calculations. Due to upstream layer of high packing density, the loading tests reveal a tendency of clogging for media M2U. Both media, M1U and M2U, exhibit better filtration performance compared to the reference media RefM and could be applied for collection of nanoparticles in HVAC system by replacing the high grade efficiency filters.