Micropore fabrics have been proposed to reduce exposure to house dust mite allergens in the field of environmental management methods. However, there is not an adequate method for verifying allergen impermeability of these micropore fabrics yet. In this study a particle penetration device was developed to test the allergen barrier performance of micropore fabrics against house dust mite allergens. The weight reduction rate of each micropore fabric was measured using the particle penetration device. At the given pressure and 90 seconds, the weight reduction ratio reached 35.6% for 6550 fabric, 13.6% 6560 fabric and 6.8% for 6563 fabric compared to 99.9% of 6060 fabric. In addition, a thermos-pressing sewing method, in which the bonding thread and the stitching portion were pressed using a thermos-pressing device, was proposed. The fabric made by the thermo-pressing sewing method effectively prevented particle penetration compared with the fabric made by the general sewing process.