Issue
Korean Journal of Chemical Engineering,
Vol.34, No.8, 2255-2264, 2017
Effects of process parameters on EPA and DHA concentrate production from Atlantic salmon by-product oil: Optimization and characterization
Supercritical carbon dioxide (SC-CO2) extracted Atlantic salmon frame bone oil (SFBO) was used for Eicosapentaenoic acid and Docosahexaenoic acid (EPA-DHA) concentrate production by urea complexation. Urea/ fatty acids (2.5 to 4.0 w/w), crystallization temperature (-24 to -8°C) and crystallization time (8 to 24 h) were studied by Box-Behnken Design (BBD) to maximize EPA-DHA content. Highest EPA-DHA content was 60.63% at urea/fatty acids 4.0 w/w, crystallization temperature -15.67 °C and crystallization time 8 h. EPA-DHA concentrate showed improvement of EPA-DHA from 6.39% in SFBO to 62.34%, increase of astaxanthin content from 21.33 μg/g in SFBO to 44.69 μg/g in EPA-DHA concentrate, no residual urea and reduction of many off-flavor compounds. The EPA-DHA yield showed an inverse relation with the urea/fatty acids, whereas its concentration increased proportionally with urea/ fatty acids. Therefore, EPA-DHA concentrate produced from SFBO by urea complexation may be an efficient technique to provide ω-3 polyunsaturated fatty acids to the consumers.
[References]
  1. Liaset B, Nortvedt R, Lied E, Espe M, Process Biochem., 37(11), 1263, 2002
  2. Deepika D, Vegneshwaran VR, Julia P, Sukhinder KC, Sheila T, Heather M, Wade M, J. Food Process Technol., 5, 1, 2014
  3. Siriwardhana N, Kalupahana NS, Moustaid MN, Adv. Food Nutr. Res., 65, 211, 2012
  4. Ruxton CHS, Reed SC, Simpson MJA, Millington K, J. Hum. Nutr. Dietet., 17, 449, 2004
  5. Haagsma N, Gent CM, Luten JB, Jong RW, Doorn E, J. Am. Oil Chem. Soc., 59, 117, 1982
  6. Gamez MN, Noriega RJA, Medina JLA, Ortega GJ, Monroy RJ, Toro VFJ, Garcia HS, Angulo O, Food Res. Int., 36, 721, 2003
  7. Ratnayake WMN, Olsson B, Matthews D, Ackman RG, Fat Sci. Technol., 90, 381, 1988
  8. Senanayake SPJN, Shahidi F, J. Food Lipids, 7, 51, 2000
  9. Anon, Fish oil production and potentials, In Atlantic Canada’s Project Report. No. 115, Fisheries Development Branch, Halifax, NS, Canada (1986).
  10. Singh AK, Mukhopadhyay M, Korean J. Chem. Eng., 33(4), 1247, 2016
  11. Rubio-Rodriguez N, de Diego SM, Beltran S, Jaime I, Sanz MT, Rovira J, J. Supercrit. Fluids, 47(2), 215, 2008
  12. Wanasundara UN, Shahidi F, Food Chem., 65, 41, 1999
  13. Bas D, Boyaci IH, J. Food Eng., 78(3), 836, 2007
  14. Gan CY, Latiff AA, Food Chem., 124, 1277, 2011
  15. Bezerra MA, Santelli RE, Oliveira EP, Villar LS, Escaleira LA, Talanta, 76, 965, 2008
  16. Mason RL, Gunst RF, Hess JL, Wiley, New York, U.S.A. (1989).
  17. Derringer G, J. Qual. Technol., 12, 214, 1980
  18. AOCS Official Method Ce 1-62. AOCS Press, Champaign, Illinois, U.S.A. (2006).
  19. Ali-Nehari A, Kim SB, Lee YB, Lee H, Chun BS, Korean J. Chem. Eng., 29(3), 329, 2012
  20. Ramesh KB, Khushboo KG, Valecha P, Shradda PK, Sushma M, Int. J. Eng. Res. Gen. Sci., 3, 809, 2015
  21. Lee MK, Uddin MS, Chun BS, J. Envrion. Biol., 29, 591, 2008
  22. Ghosh PK, Bhattacharjee P, Korean J. Chem. Eng., 33(5), 1681, 2016
  23. Liu SC, Zhang CH, Hong PZ, Ji HW, J. Food Eng., 73(3), 203, 2006
  24. Chin YF, Salimon J, Said M, Sains Malays., 39, 795, 2010
  25. Myers RH, Montgomery DC, Anderson-Cook CM, Wiley (2009).
  26. Swern D, New York, U.S.A. (1964).
  27. Schlenk H, pp. 243-267, Pergamon Press, London, UK (1954).
  28. Medina RA, Gimnez A, Camacho GF, Perez SJA, Grima ME, Gomez CA, J. Am. Oil Chem. Soc., 72, 575, 1995
  29. Rodriguez A, Esteban L, Martin L, Jimenez MJ, Hita E, Castillo B, Gonzalez PA, Robles A, Enzyme Microb. Technol., 51(3), 148, 2012
  30. Esteban L, Munio MM, Robles A, Hita E, Jimenez MJ, Gonzalez PA, Camacho B, Molina E, Biochem. Eng. J., 44, 271, 2009
  31. Suriani NW, Lawalata HJ, Komansilan A, Int. J. Pharm. Technol. Res., 6, 1981, 2014
  32. Lee YR, Baokun T, Kyung HR, Asian J. Chem., 26, 4543, 2014
  33. Zhou J, Bi W, Row KH, J. Food Sci., 76, 441, 2011
  34. Fassett RG, Coombes JS, Molecules, 17, 2030, 2012
  35. Hsleh TCY, Williams SS, Vejaphan W, Meyers SP, J. Am. Oil Chem. Soc., 66, 112, 1989
  36. Mathews RF, Scanlon RA, Libbey LM, J. Am. Oil Chem. Soc., 48, 745, 1971
  37. Hu SP, Pan BS, J. Am. Oil Chem. Soc., 77, 41, 2000