Indiscriminate use of antibiotics and the rise in drug resistance emphasizes the immediate need for managing pharmaceutical products sensibly. The pharmaceutical products end in wastewater due to the manufacturing process of pharmaceutical industries, misuse of antibiotics and improper disposal of expired drugs, which eventually ends in sewers. In the present study we attempted to degrade fluoroquinolones by the process of mycoremediation. Hospital waste water soil sample was collected and screened for fungi which can tolerate norfloxacin. One fungal isolate was able to withstand 2 g/L of norfloxacin which was chosen for degradation studies. Degradation pattern was checked with HPLC, FTIR and SEM analysis. Kinetics studies were carried out to analyze the degradation pattern. The result confirmed that Ganoderma lucidum JAPC1 strain was capable of degrading norfloxacin successfully but was unable to degrade ciprofloxacin.