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Issue
Korean Journal of Chemical Engineering,
Vol.31, No.9, 1616-1623, 2014
Chitosan-based bioglass composite for bone tissue healing: Oxidative stress status and antiosteoporotic performance in a ovariectomized rat model
Jebahi S, Oudadesse H, Saleh GB, Saoudi M, Mesadhi S, Rebai T, Keskes H, el Feki A, el Feki H
Tissue engineering has opened up a new therapeutic avenue promising a revolution in regenerative medicine. Considerable attention has been given to chitosan composite materials and their applications in the field of the bone graft substitutes. We evaluated the antioxidative properties of chitosan-doped bioactive glass (BG-CH) with 17 wt% chitosan, and their applications in the guided bone regeneration. BG-CH was produced by a freeze-drying process and implanted in the femoral condyles of ovariectomized rats. Grafted bone tissues were carefully removed to evaluate the oxidative stress analysis, histomorphometric profile and mineral bone distribution by using inductively coupled plasma optical emission spectrometry (ICP-OES). A significant decrease of thiobarbituric acid-reactive substances (TBARs) was observed after BG-CH implantation. Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities significantly increased in ovariectomized group implanted with chitosan-doped bioactive glass (OVXBG-CH) as compared to ovariectomized group implanted with bioactive glass (OVX-BG). The histomorphometric analysis showed that bone/tissue volume (BV/TV), osteoblast number (N.Ob) and osteoblast surface/bone surface (Ob.S/BS) were significantly higher in OVX-BG-CH group than in OVX-BG group. On the other hand, a rise in Ca and P ion concentrations in the implanted microenvironment was shown to lead to the ormation/deposition of Ca-P phases. Trace elements such as Sr and Fe were detected in the newly formed bone and involved in bone healing. These results suggested that BG-CH composites could become clinically useful as a therapeutic and implantable material.
Keywords: Chitosan; Bioactive Glass; Graft Biomaterial; Osteoporosis; Antioxidative Profile; Bone Regeneration
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[Cited By]
  1. Ahn GS, Yu GS, Abdullah A, Kim YN, Lee DH, Macromolecular Research, 27(6), 579, 2019
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