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Issue
Korean Journal of Chemical Engineering,
Vol.31, No.1, 98-103, 2014
Immobilization of Candida sp.99-125 lipase onto silanized SBA-15 mesoporous materials by physical adsorption
Li Y, Wang W, Han P
SBA-15-NH2 and SBA-15-Cl Mesoporous Materials were prepared by modifying SBA-15 with silane coupling agent 3-aminopropyltriethoxysilane and 3-chloropropyltriethoxysilane using the post-synthesis method. The mesoporous samples were characterized by Fourier transform infrared spectra and nitrogen adsorption. Compared with SBA-15-NH2, SBA-15-Cl has suitable pore opening for further utilization in the immobilization of Candida sp.99-125 lipase by physical adsorption. The influences of lipase concentration and immobilizing time on the immobilization efficiency were investigated. Meanwhile, the lipase immobilized on SBA-15-Cl showed higher thermal, pH and storage stability than that of free lipase. Further study demonstrated that lipase immobilized on SBA-15-Cl could be used eight times without significant decrease of enzyme activity. The phenomenon was associated with the shrinkage of the pore opening of SBA-15-Cl keeping lipase from leaping out.
Keywords: Silanization; SBA-15; Lipase; Immobilization; Stability; Reusability
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[Cited By]
  1. Yu WH, Zhao HB, Tong DS, Zhou CH, Shao P, Korean Journal of Chemical Engineering, 32(8), 1694, 2015
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