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Issue
Korean Journal of Chemical Engineering,
Vol.30, No.9, 1775-1783, 2013
Chitosan effects on glass matrices evaluated by biomaterial. MAS-NMR and biological investigations
Oudadesse H, Wers E, Bui XV, Roiland C, Bureau B, Akhiyat I, Mostafa A, Chaair H, Benhayoune H, Faure J, Pellen-Mussi P
Bioactive glass 46S6 and biodegradable therapeutic polymer (Chitosan: CH) have been elaborated to form 46S6-CH composite by freeze-drying process. The kinetics of chemical reactivity and bioactivity at the surface were investigated by using physicochemical techniques, particularly solid-state MAS-NMR. Immortalized cell line used to construct multicellular spheroids was employed as three-dimensional (3D) cell cultures for in vitro studies. Obtained results showed a novel structure of the composite; the chemical treatment (ultrasound, magnetic stirring, freeze drying process and lyophilization) led the bioactive glass particles to be loaded in the chitosan-based materials. 29Si and 31P MAS-NMR results showed the emergence of two new species, QSi3(OH) and QSi4, which are characteristic of the vitreous network dissolution in simulated body fluid (SBF). MAS-NMR also confirmed the formation of amorphous calcium phosphate (ACP) at the surface of the initial 46S6-CH. Three-dimensional (3D) cell cultures highlighted the effect of chitosan, where the cell viability reached up to 78% in 46S6-CH composite and up to 67% in 46S6. The association of (CH) and bioactive glass (BG) matrix promotes a highly significant bioactivity, demonstrating surface bone formation and satisfactory behavior in biological environment.
Keywords: Bioactive Glass; Chitosan; Composite; Bioactivity; Hydroxyapatite; 31P MAS NMR; 29Si MAS NMR; Immortalized Cell; 3D Cells Viability
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[Cited By]
  1. Lee KR, Song KH, Korean Journal of Chemical Engineering, 31(1), 162, 2014
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