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Korean Journal of Chemical Engineering, Vol.25, No.6, 1473-1476, 2008
Protein binding study of catechin hydrate and genistein by high-performance frontal analysis
High-performance frontal analysis (HPFA) was used for the protein binding study of catechin hydrate and genistein to human serum albumin (HSA). The experiment was performed on a Develosil 100Diol-5 column, and sodium phosphate buffer (pH 7.4 and ionic strength of 0.17) was used as the mobile phase. The mixtures of the drug-HSA solution were directly injected into the HPFA column, the HSA was eluted first and the unbound drugs were eluted out as a trapezoidal peak with a plateau region. The unbound drug concentration was determined from a plateau height of the plateau region and the experimental data were fitted by Scatchard equation. The binding constants (K) and binding affinities (nK) of the drug to HAS were K=1.32×10^(4) (L mol^(-1)), nK=0.47×10^(4) (L mol^(-1)) for catechin hydrate, and K=5.17×10^(4) (L mol^(-1)), nK=2.14×10^(4) (L mol^(-1)) for genistein.
[References]
- Meyer MC, Guttman DE, Journal of Pharmaceutical Science, 57, 895, 1968
- Kwong TC, Clinica Chimica Acta., 151, 193, 1985
- Svensson CK, Woodruff MN, Baxter JG, Lalka D, Clinical Pharmacokinet, 11, 450, 1986
- Eriksson MAL, Gabrielsson J, Nilsson LB, Journal of Pharmaceutical and Biomedical Analysis, 38, 381, 2005
- Hoyo PD, Moure F, Rendueles M, Diaz M, Meat Science, 76, 402, 2007
- Chan ACK, Lelj-Garolla B, Rosell FI, Pedersen KA, Mauk AG, Murphy MEP, Journal of Molecular Biology, 362, 1108
- Adebowale YA, Adeyemi IA, Oshodi AA, Niranjan K, Food Chemistry, 104, 287, 2007
- Shibukawa A, Kuroda Y, Nakagawa T, Journal of Pharmaceutical T. Nakagawa, Journal of Pharmaceutica, 18, 1047, 1999
- Qiao M, Guo X, Li F, Journal of Chromatography A, 952, 131, 2002
- Shibukawa A, Sawada T, Nakao C, Izumi T, Nakagawa T, Journal of Chromatography A, 697, 337, 1995
- Shibukawa A, Nakagawa T, Analytical Chemistry, 68, 447, 1996
- Choi DY, Jin LM, Wang D, Row KH, Korean J. Chem. Eng., 22(3), 465, 2005
- Rosas MER, Shibukawa A, Ueda K, Nakagawa T, Journal of Pharmaceutical and Biomedical Analysis, 15, 1595, 1997
- Yazawa K, Kihara T, Shen H, Shimmyo Y, Niidome T, Sugimoto H, FEBS Letters, 580, 6623, 2006
- Jullian C, Miranda S, Zapata-Torres G, Mendizabal F, Olea-Azar C, Bioorganic & Medicinal Chemistry, 15, 3217, 2007
- El-Hady DA, Analytica Chimical Acta, 593, 178, 2007
- Wu H, Chan W, Toxicology in Vitro, 21, 335, 2007
- Chodon D, Ramamurty N, Sakthisekaran D, Toxicology in Vitro, 21, 887, 2007
- Vergne S, Titier K, Bernard V, Asselineau J, Durand M, Lamothe V, Potier M, Perez P, Demotes-Mainard J, Chantre P, Moore N, Bennetau-Pelissero C, Sauvant P, Journal of Pharmaceutical and Biomedical Analysis, 43, 1488, 2007
- Jin L, Choi D, Liu H, Row KH, Bulletin of Korean Chemical Society, 26, 136, 2005
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