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Korean Journal of Chemical Engineering, Vol.20, No.3, 538-541, 2003
Separation of Whey Proteins by Anion-Exchange Membranes
Three strong anion-exchange membranes (CIM QA, Q100 and HiTrap Q) were investigated for the
separation of the major proteins, which were contained in whey, such as α-Lactalbumin, BSA and β-Lactoglobulin. Experiments were performed to determine the optimum mobile phase composition for separating the whey proteins using the standard chemicals of the proteins. The mobile phase was buffer A (20 mM piperazine-HCl pH 6.4) and buffer B (buffer A+1 M NaCl) and the linear gradient elution changes of salt concentration were applied. The standard chemicals of the proteins were used to investigate the optimal mobile phase compositions with the three anion-exchange membranes. From the experimental results, it was found that HiTrap Q was the most effective in separating whey proteins.
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[Cited By]
- Jung YA, Choi DY, Hong SB, Row KH, Korean Journal of Chemical Engineering, 20(4), 705, 2003
- Demirhan E, Apar DK, Ozbek B, Korean Journal of Chemical Engineering, 27(2), 536, 2010
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- Yoon HM, Lee EJ, Lim KH, Korean Chemical Engineering Research, 56(6), 832, 2018
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