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Korean Journal of Chemical Engineering, Vol.29, No.2, 209-214, 2012
Bioethanol production from micro-algae, Schizocytrium sp., using hydrothermal treatment and biological conversion
Hydrothermal fractionation for micro-algae, Schizocytrium sp., was investigated to separate sugars, lipids, and proteins. This fractionation process produced protein-rich solid cake and liquid hydrolysates, which contained oligomeric sugars and lipids. Oligomeric sugars and lipids were easily separated by liquid-liquid separation. Sugars in the separated hydrolyzate were determined to be mainly D-glucose and L-galactose. Fractionation conditions were optimized
by response surface methodology (RSM). Optimal conditions were found to be 115.5 ℃ of reaction temperature, 46.7min of reaction time, and 25% (w/w) of solid loading. The model predicted that maximum oligomeric sugar yield (based on untreated micro-algae weight), which can be recovered by hydrothermal fractionation at the optimum conditions, was 19.4 wt% (based on the total biomass weight). Experimental results were in agreement with the model prediction of 16.6 wt%. Production of bioethanol using micro-algae-induced glucan and E. coli KO11 was tested with SSF (simultaneous saccharification and fermentation), which resulted in 11.8 g-ethanol/l was produced from 25.7 g/l of glucose; i.e. the theoretical maximum ethanol yield based on glucan in hydrolyzate was 89.8%.
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[Cited By]
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- Park JY, Lee GA, Kim KY, Kim KY, Choi SA, Jeong MJ, Oh YK, Korean Chemical Engineering Research, 52(1), 88, 2014
- Choi KS, Ryu JH, Park DJ, Oh SC, Kwak H, Korean Chemical Engineering Research, 53(2), 205, 2015
- Kim J, Ha SH, Korean Chemical Engineering Research, 53(5), 570, 2015
- Kim G, Lee CH, Lee K, Korean Journal of Chemical Engineering, 33(1), 230, 2016
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