Print: | ISSN 0256-1115 |
Online: | ISSN 1975-7220 |
Korean Journal of Chemical Engineering,
Vol.19, No.5, 780-782, 2002 Analysis of Microbial Adaptation at Enzyme Level for Enhancing Biodegradation Rate of BTX
Catechol was found to be a common intermediate in the degradation of benzene and toluene by Alcaligenes xylosoxidans Y234, and the ring cleavage of the catechol mediated by catechol 1,2-dioxygenase was a rate-determining step. Since benzene induced higher level of catechol 1,2-dioxygenase than toluene, the cells pre-adapted to benzene showed higher degradation rate of benzene and toluene. The degradation rate of m-xylene was also increased significantly when benzene-adapted cells were inoculated. m-Xylene was metabolized via 3-methyl catechol which was effectively cleaved by catechol 1,2-dioxygenase.
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