|
Korean Journal of Chemical Engineering, Vol.37, No.3, 482-492, 2020
Antifungal mechanisms of ε-poly-L-Lysine with different molecular weights on Saccharomyces cerevisiae
ε-Poly-L-lysine (ε-PL) is a natural antimicrobial cationic peptide. Antimicrobial activity of ε-PL is closely related to its molecular weight (Mw). However, the antimicrobial mechanisms of ε-PL with different Mws are still vague. In this study, Saccharomyces cerevisiae was used as the model system to analyze the mechanism from these three aspects: cell wall, cell membrane, and metabolism. The results showed that high-Mw ε-PL (1-3 kDa and >3 kDa) and commercial ε-PL product caused cell wall lesions, and significantly improved cell membrane permeability compared to low-Mw ε-PL (<1 kDa), resulting in leaking of the protoplasm through the pores and cell death. Furthermore, metabolomics analysis showed that high-Mw ε-PL (1-3 kDa and >3 kDa) and product displayed higher inhibition effect on the glycolysis pathway and tricarboxylic cycle than that of low-Mw ε-PL (<1 kDa).
[References]
- Gould G, Russell N, Food preservatives, Springer (2003).
- Ritchie ML, Romanuk TN, Plos One, 7, e34938, 2012
- Gao C, Tao Y, Jie D, Fang H, Luo H, Wan Y, Food Hydrocolloids, 36, 204, 2014
- Xu Z, Xu Z, Feng X, Xu D, Liang J, Xu H, Appl. Microbiol. Biotechnol., 100, 15, 2016
- Miao JY, Zhou JL, Liu G, Chen FL, Chen Y, Gao X, Dixon W, Song M, Xiao H, Cao Y, Food Control, 59, 609, 2016
- Hyldgaard M, Mygind T, Vad BS, Stenvang M, Otzen DE, Meyer RL, Appl. Environ. Microbiol., 80, 7759, 2014
- Su R, Li T, Fan D, Huang J, Zhao J, Yan B, Zhou W, Zhang W, Zhang H, J. Sci. Food Agric., 99, 6, 2019
- Shima S, Matsuoka H, Iwamoto T, Sakai H, J. Antibiot., 37, 11, 1984
- Takehara M, Hibino A, Saimura M, Hirohara H, Biotechnol. Lett., 32, 9, 2010
- Wei M, Ge Y, Li C, Chen Y, Wang W, Duan B, Li X, Physiol. Mol. Plant Pathol., 103, 23, 2018
- Ye R, Xu H, Wan C, Peng S, Wang L, Xu H, Aguilar ZP, Xiong Y, Zeng Z, Wei H, Biochem. Biophys. Res. Commun., 439, 1, 2013
- Liu K, Zhou X, Fu M, Postharvest Biol. Tec., 123, 94, 2017
- Li H, He C, Li G, Zhang Z, Li B, Tian S, Postharvest Biol. Technol., 147, 1, 2019
- Wang E, Li Y, Maguy BL, Lou Z, Wang H, Zhao W, Chen X, Food Chem., 294, 533, 2019
- Veerman ECI, Valentijn-Benz M, van’t Hof W, Nazmi K, van Marle J, Amerongen AVN, Biol. Chem., 391, 1, 2010
- Zhang S, Xiong J, Lou W, Ning Z, Zhang D, Yang J, Przem. Chem., 98(1), 113, 2019
- Ding MZ, Zhou X, Yuan Y, Metabolomics, 6, 1, 2010
- Shih IL, Shen MH, Van YTJBT, Bioresour. Technol., 97, 9, 2006
- Song R, Wei RB, Luo HY, Wang DFJM, Molecules, 17, 3, 2012
- Guilhelmelli F, Vilela N, Albuquerque P, Derengowski LS, Silva-Pereira I, Kyaw C, Front Microbiol., 4, 353, 2013
- Yeaman MR, Yount NY, Pharmacol. Rev., 55, 1, 2003
- Ladokhin AS, White SHJBB, BBA-Biomembranes, 1514, 2, 2001
- Teixeira V, Feio MJ, Bastos M, Prog. Lipid Res., 51, 2, 2012
- Brogden KA, Nat. Rev. Microbiol., 3, 3, 2005
- Singer MA, Lindquist S, Trends in Biotechnol., 16, 11 (1998).
- Sharma SC, FEMS Microbiol. Lett., 152, 11, 1997
- Parrou JL, Teste MA, Francois J, J. Microbiol., 143, 6, 1997
- Godon C, Lagniel G, Lee J, Buhler JM, Kieffer S, Perrot R, Boucherie H, Toledano MB, Labarre J, J. Biol. Chem., 273, 35, 1998
- Natera V, Sobrevals L, Fabra A, Castro S, Curr. Microbiol., 53, 6, 2006
- Yap S, Lim SJAOM, Arch Microbiol., 135, 3, 1983
- Takagi H, Iwamoto F, Nakamori S, Appl. Microbiol. Biotechnol., 47, 4, 1997
- Kubota S, Takeo I, Kume K, Kanai M, Shitamukai A, Mizunuma M, Miyakawa T, Shimoi H, Iefuji H, Hirata D, Biosci. Biotechnol. Biochem., 68, 4, 2004
- Li H, Ma M, Luo S, Zhang R, Han P, Hu W, Int. J. Biochem. Cell B., 44, 7, 2012
- Ding M, Wang X, Yang Y, Yuan Y, OMICS: J. Integrative Biol., 15, 10, 2011
- Barsch A, Carvalho HG, Cullimore JV, Niehaus K, J. Biotechnol., 127, 1, 2006
|