Korean Journal of Chemical Engineering, Vol.39, No.5, 1097-1106, 2022
Energy metabolism in Chinese hamster ovary (CHO) cells: Productivity and beyond
Chinese hamster ovary (CHO) cell lines have been widely used to produce recombinant proteins. While the biosynthesis of recombinant proteins is energy-intensive, CHO cells exhibit inefficient metabolism, characterized by rapid conversion of glucose to lactate, possibly leading to lower cell growth and productivity of therapeutic proteins. Therefore, it is important to understand and engineer cellular metabolism to increase recombinant protein production. In this review, cellular energy metabolism of CHO cells with respect to protein synthesis is overviewed. Then, genetic and process engineering approaches to enhance metabolic efficiency are described, resulting in the improvement of cell culture performance. Finally, recent modeling technologies for understanding and predicting cellular metabolic behaviors are reviewed. These efforts will aid to further advance the biomanufacturing of therapeutic proteins.
[References]
Walsh G, Nat. Biotechnol. , 36 , 1136, 2018
Grilo AL, Mantalaris A, Trends Biotechnol. , 37 , 9, 2019
Zhu MM, Mollet M, Hubert RS, Kyung YS, Zhang GG, Handbook of industrial chemistry and biotechnology, 3rd edition, Springer (2017).
Lai T, Yang Y, Ng SK, Pharmaceuticals , 69 , 579, 2013
Pereira S, Kildegaard HF, Andersen MR, Biotechnol. J. , 13 , e1700499, 2018
Rosano GL, Ceccarelli EA, Front. Microbiol. , 5 , 172, 2014
Henry MN, MacDonald MA, Orellana CA, Gray PP, Gillard M, Baker K, Nielsen LK, Marcellin E, Mahler S, Martínez VS, Biotechnol. Bioeng. , 117 , 1187, 2020
Hartley F, Walker T, Chung V, Morten K, Biotechnol. Bioeng. , 115 , 1890, 2018
Horvat J, Narat M, Spadiut O, Biotechnol. Prog. , 36 , e3001, 2020
Ivarsson M, Noh H, Morbidelli M, Soos M, Biotechnol. Prog. , 31 , 347, 2015
Cacciatore JJ, Chasin LA, Leonard EF, Biotechnol. Adv. , 28 , 673, 2010
Gonzalez MG, Latorre Y, Zuniga R, Aguillon JC, Molina MC, Altamirano C, Crit. Rev. Biotechnol. , 39 , 665, 2019
Blondeel EJM, Aucoin MG, Biotechnol. Adv. , 36 , 1505, 2018
Beyer B, Schuster M, Jungbauer A, Lingg N, Biotechnol. J. , 13 , 1700476, 2018
Martinez VS, Dietmair S, Quek LE, Hodson MP, Gray P, Nielsen LK, Biotechnol. Bioeng. , 110 , 660, 2013
Young JD, Curr. Opin. Biotechnol. , 24 , 1108, 2013
Rich PR, Biochem. Soc. Trans. , 31 , 1095, 2003
Romanova N, Niemann T, Greiner JFW, Kaltschmidt B, Kaltschmidt C, Noll T, Biotechnol. Bioeng. , 118 , 2348, 2021
Alhuthali S, Kotidis P, Kontoravdi C, Int. J. Mol. Sci. , 22 , 3290, 2021
Lieske PL, Wei W, Crowe KB, Figueroa B, Zhang L, Biotechnol. J. , 15 , e1900306, 2020
Liberti MV, Locasale JW, Trends Biochem. Sci. , 41 , 211, 2016
Heiden MGV, Cantley LC, Thompson CB, Science , 324 , 1029, 2009
Warburg O, Cancer Res. , 9 , 148, 1925
Hume DA, Radik JL, Ferber E, Weidemann MJ, Biochem. J. , 174 , 703, 1978
DeBerardinis RJ, Mancuso A, Daikhin E, Nissim I, Yudkoff M, Wehrli S, Thompson CB, Proc. Natl. Acad. Sci. , 104 , 19345, 2007
Lunt SY, Heiden MGV, Annu. Rev. Cell Dev. Biol. , 27 , 441, 2011
Zu XL, Guppy M, Biochem. Biophys. Res. Commun. , 313 , 459, 2004
Locasale JW, Cantley LC, Cell Metab. , 14 , 443, 2011
Zagari F, Jordan M, Stettler M, Broly H, Wurm FM, N. Biotechnol. , 30 , 238, 2013
Ghorbaniaghdam A, Chen J, Henry O, Jolicoeur M, PLoS One , 9 , e90832, 2014
Ma N, Ellet J, Okediadi C, Hermes P, McCormick E, Casnocha S, Biotechnol. Prog. , 25 , 1353, 2009
Wahrheit J, Niklas J, Heinzle E, Metab. Eng. , 23 , 9, 2014
Sun YT, Zhao L, Ye Z, Fan L, Liu XP, Tan WS, Biochem. Eng. J. , 81 , 126, 2013
Le H, Kabbur S, Pollastrini L, Sun Z, Mills K, Johnson K, Karypis G, Hu WS, J. Biotechnol. , 162 , 210, 2012
Templeton N, Dean J, Reddy P, Young JD, Biotechnol. Bioeng. , 110 , 2013, 2013
Yong J, Bischof H, Burgstaller S, Siirin M, Murphy A, Malli R, Kaufman RJ, Elife , 8 , e49682, 2019
Klein MC, Zimmermann K, Schorr S, Landini M, Klemens PAW, Altensell J, Jung M, Krause E, Nguyen D, Helms V, Nat. Commun. , 9 , 3489, 2018
Yoo HC, Park SJ, Nam M, Kang J, Kim K, Yeo JH, Kim JK, Heo Y, Lee HS, Lee MY, Lee CW, Kang JS, Kim YH, Cell Metab. , 31 , 267, 2020
Yoo HC, Yu YC, Sung Y, Han JM, Exp. Mol. Med. , 52 , 1496, 2020
Budge JD, Knight TJ, Povey J, Roobol J, Brown IR, Singh G, Dean A, Turner S, Jaques CM, Young RJ, Racher AJ, Smales CM, Metab. Eng. , 57 , 203, 2020
Li W, Fan Z, Lin Y, Wang TY, Front. Bioeng. Biotechnol. , 9 , 646363, 2021
Houten SM, Violante S, Ventura FV, Wanders RJ, Annu. Rev. Physiol. , 78 , 23, 2016
Gutierrez JM, Feizi A, Li S, Kallehauge TB, Hefzi H, Grav LM, Ley D, Hizal DB, Betenbaugh MJ, Voldborg B, Kildegaard HF, Nat. Commun. , 11 , 68, 2020
Hiller K, Grote A, Scheer M, Munch R, Jahn D, Nucleic Acids Res. , 32 , 375, 2004
Fankhauser N, Maser P, Bioinformatics , 21 , 1846, 2005
Yang Z, Halim A, Narimatsu Y, Joshi HJ, Steentoft C, Schjoldager KTBG, Schulz MA, Sealover NR, Kayser KJ, Mol. Cell Proteomics. , 13 , 3224, 2014
Kapp LD, Lorsch JR, Annu. Rev. Biochem. , 73 , 657, 2004
Noh SM, Sathyamurthy M, Lee GM, Curr. Opin. Chem. Biol. , 2 , 391, 2013
Tihanyi B, Nyitray L, Drug Discov. Today , 38 , 25, 2021
Reinhart LDD, Sommeregger W, Gili A, Schafellner S, Castan A, Kaisermayer C, Kunert R, BMC Proc. , 9 , 36, 2015
Kim SH, Lee GM, Appl. Microbiol. Biotechnol. , 74 , 152, 2007
Jeon MK, Yu DY, Lee GM, Appl. Microbiol. Biotechnol. , 92 , 779, 2011
Noh SM, Park JH, Lim MS, Kim JW, Lee GM, Appl. Microbiol. Biotechnol. , 101 , 1035, 2017
Irani N, Wirth M, Heuvel JVD, Wagner R, Biotechnol. Bioeng. , 66 , 238, 1999
Vallee C, Durocher Y, Henry O, J. Biotechnol. , 169 , 63, 2014
Toussaint C, Henry O, Durocher Y, J. Biotechnol. , 217 , 122, 2016
Gupta SK, Srivastava SK, Sharma A, Nalage VHH, Salvi D, Kushwaha H, Chitnis NB, Shukla P, PLoS One , 12 , e0181455, 2017
Gupta SK, Sharma A, Kushwaha H, Shukla P, Front. Pharmacol. , 8 , 463, 2017
Wlaschin KF, Hu WS, J. Biotechnol. , 131 , 168, 2007
Leong DS, Tan JC, Chin CL, Mak SY, Ho YS, Ng SK, Sci. Rep. , 7 , 45216, 2017
Leong DSZ, Teo BKH, Tan JGL, Kamari H, Yang YS, Zhang P, Ng SK, Sci. Rep. , 8 , 4037, 2018
Wilkens CA, Altamirano C, Gerdtzen ZP, Biotechnol. Bioprocess Eng. , 16 , 714, 2011
Torres M, Berrios J, Rigual Y, Latorre Y, Vergara M, Dickson AJ, Altamirano C, Chem. Eng. Sci. , 205 , 201, 2019
Buchsteiner M, Quek LE, Gray P, Nielsen LK, Biotechnol. Bioeng. , 115 , 2315, 2018
Moller J, Bhat K, Guhl L, Portner R, Jandt U, Zeng AP, Eng. Life Sci. , 21 , 100, 2021
Urquiza LT, Martin AEA, James DC, Nagy T, Falconer RJ, Biotechnol. Prog. , 36 , e2940, 2020
Scorrano L, De Matteis MA, Emr S, Giordano F, Hajnóczky G, Kornmann B, Lackner LL, Levine TP, Pellegrini L, Reinisch K, Nat. Commun. , 10 , 1287, 2019
Calleja LL, Lecina M, Repullo JL, Albiol J, Sola C, Cairo JJ, Appl. Microbiol. Biotechnol. , 99 , 9951, 2015
Zalai D, Koczka K, Parta L, Wechselberger P, Klein T, Herwig C, Biotechnol. Prog. , 31 , 1657, 2015
Osman JBJJ, Varley J, Biotechnol. Bioeng. , 79 , 398, 2002
Brunner M, Doppler P, Klein T, Herwig C, Fricke J, Eng. Life Sci. , 18 , 204, 2018
Gagnon M, Hiller G, Luan YT, Kittredge A, DeFelice J, Drapeau D, Biotechnol. Bioeng. , 108 , 1328, 2011
Mulukutla BC, Kale J, Kalomeris T, Jacobs M, Hiller GW, Biotechnol. Bioeng. , 114 , 1779, 2017
Calmels C, McCann A, Malphettes L, Andersen MR, Metab Eng. , 51 , 9, 2019
Yahia BB, Malphettes L, Heinzle E, Appl. Microbiol. Biotechnol. , 99 , 7009, 2015
Huang Z, Lee DY, Yoon S, Biotechnol. Bioeng. , 114 , 2717, 2017
Kyriakopoulos S, Ang KS, Lakshmanan M, Huang Z, Yoon S, Gunawan R, Lee DY, Biotechnol. J. , 13 , e1700229, 2018
Tang P, Xu J, Louey A, Tan Z, Yongky A, Liang S, Li ZJ, Weng Y, Liu S, Crit. Rev. Biotechnol. , 40 , 265, 2020
Sha S, Huang Z, Wang Z, Yoon S, Curr. Opin. Chem. Eng. , 22 , 54, 2018
Islam MM, Schroeder WL, Saha R, Curr. Opin. Syst. Biol. , 26 , 72, 2021
Stalidzans E, Seiman A, Peebo K, Komasilovs V, Pentjuss A, Biochem. Soc. Trans. , 46 , 261, 2018
Gutierrez JM, Lewis NE, Biotechnol. J. , 10 , 939, 2015
Rejc Z, Magdevska L, Trselic T, Osolin T, Vodopivec R, Mraz J, Pavliha E, Zimic N, Cvitanović T, Rozman D, Moškon M, Comput. Biol. Med. , 88 , 150, 2017
Altamirano C, Illanes A, Becerra S, Cairo JJ, Godia F, J. Biotechnol. , 125 , 547, 2006
Sheikholeslami Z, Jolicoeur M, Henry O, Biotechnol. Prog. , 30 , 535, 2014
O'Brien CM, Zhang Q, Daoutidis P, Hu WS, Metab Eng. , 66 , 31, 2021
Yahia BB, Malphettes L, Heinzle E, Metab Eng. , 66 , 204, 2021
Selvarasu S, Kim DY, Karimi IA, Lee DY, J. Biotechnol. , 150 , 94, 2010
Zhanga Y, Lin H, Yanga Z, Wanga J, Sun Y, Xu B, Zhao Z, J. Biomed. Inform. , 99 , 103294, 2019
Schmitt J, Downey B, Beller J, Russell B, Quach A, Lyon D, Curran M, Mulukutla BC, Chu C, Biotechnol. Bioeng. , 116 , 2223, 2019
Schinn SM, Morrison C, Wei W, Zhang L, Lewis NE, Biotechnol. Bioeng. , 118 , 2118, 2021
Huang Z, Xu J, Yongkyb A, Morrisa CS, Polancoa AL, Reilyc M, Borysb MC, Lib ZJ, Yoon S, Biochem. Eng. J. , 160 , 1076938, 2020
Calmels C, Arnoult S, Yahia BB, Malphettes L, Andersen MR, Metab. Eng. , 9 , e00097, 2019
Huang Z, Yoon S, Processes , 8 , 331, 2020
Luo Y, Lovelett RJ, Price JV, Radhakrishnan D, Barnthouse K, Hu P, Schaefer E, Cunningham J, Lee KH, Shivappa RB, Ogunnaike BA, Biotechnol. J. , 16 , e2000261, 2021
van Rosmalen RP, Smith RW, dos Santos VAPM, Fleck C, Suarez-Diez M, Metab. Eng. , 64 , 74, 2021
Yeo HC, Hong J, Lakshmanan M, Lee DY, Metab. Eng. , 60 , 138, 2020
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