Korean Journal of Chemical Engineering, Vol.26, No.4, 1075-1083, 2009
Production of polysaccharides by submergedmycelial culture of entomopathogenic fungus Cordyceps takaomontana and their apoptotic effects on human neuroblastoma cells
The effect of medium components and environmental factors on the production of mycelial biomass for the preparation of intracellular polysaccharides (IPS) and exopolysaccharides (EPS) by Cordyceps takaomontan was investigated in submerged cultures. The optimal culture condition was as follows (g/l): glucose 30, yeast extract 4, KH2PO4 0.46, K2HPO4 1, and MgSO4 0.5, and 28 ℃ and pH 8. When the fungus was cultivated under various agitation and aeration conditions in a 5-l stirred-tank fermenter, the maximum mycelial biomass (10.7 g/l) and EPS production (1.9 g/l) was obtained at 300 rpm and 2 vvm, respectively. The inhibitory effect of both IPS and EPS on the growth of SKN-SH cells was studied by treating the cells with crude IPS and EPS at concentrations of 0.5, 1, and 2 mg/ml for 24, 48, 72 h, respectively. The maximum inhibitory effect on apoptosis of SK-N-SH cells (64.6%) was observed when IPS was treated at a concentration of 2 mg/ml, for 72 h. The apoptosis of SK-N-SH cells induced by IPS was evidenced by comet assay, where the number of the comet cells increased by the IPS treated. These findings suggest that IPS may therefore have therapeutic potential against neuroblastoma cells.
[References]
Nikoh N, Fukatsu T, Mol. Biol. Evol. , 17 , 629, 2000
Kobayasi Y, Botanical Institute, Tokyo, 1941
Samson RA, Centralbureau voor Schimmelcultures, Baarn, 1974
Koh JH, Kim JM, Chang UJ, Suh HJ, Biol. Pharm. Bull. , 26 , 84, 2003
Chen W, Zhao Z, Chen SF, Li YQ, Bioresour. Technol. , 99 , 3187, 2008
Mao XB, Eksriwong T, Chauvatcharin S, Zhong JJ, Process Biochem. , 40 , 1667, 2005
Kim SW, Hwang HJ, Xu CP, Sung JM, Choi JW, Yun JW, J. Appl. Microbiol. , 94 (1), 120, 2003
Park JP, Kim YM, Kim SW, Hwang HJ, Cho YJ, Lee YS, Song CH, Yun JW, Process Biochem. , 37 , 1257, 2002
Xiao JH, Chen DX, Liu JW, Liu ZL, Wan WH, Fang N, Xiao Y, Qi Y, Liang ZQ, J. Appl. Microbiol. , 96 (5), 1105, 2004
Xiao JH, Chen DX, Xiao Y, Liu ZL, Wan WH, Fang N, Tan BB, Liang ZQ, Liu AY, Process Biochem. , 39 , 2241, 2004
Daba AS, Ezeronye OU, Afr. J. Biotechnol. , 2 , 672, 2003
Levander F, Radstrom P, Appl. Environ. Microbiol. , 67 , 2734, 2001
Looijestenijn PJ, Boels IC, Kleerebezem M, Hugenholtz J, Appl. Environ. Microbiol. , 65 , 5003, 1999
Wasser SP, Appl. Microbiol. Biotechnol. , 60 (3), 258, 2002
Bok JW, Lermer L, Chilton J, Klingeman HG, Towers GH, Phytochemistry , 51 , 891, 1999
Leung PH, Zhang QX, Wu JY, J. Appl. Microbiol. , 101 (2), 275, 2006
Toth JO, Luu B, Ourisson G, Tetrahedron Lett. , 24 , 1081, 1983
Wu WC, Hsiao JR, Lian YY, Lin CY, Huang BM, Cancer. Chemother. Pharmacol. , 60 , 103, 2007
Yap AT, Ng ML, Int. J. Med. Mushrooms. , 5 , 339, 2003
Kim GY, Roh SI, Park SK, Ahn SC, Oh YH, Lee JD, Park YM, Biol. Pharm. Bull. , 26 , 1418, 2003
Bae JT, Sinha J, Park JP, Song CH, Yun JW, J. Microbiol. Biotechnol. , 10 , 482, 2000
Kim YO, Park HW, Kim JH, Lee JY, Moon SH, Shin CS, Life Sci. , 79 , 72, 2006
Cho EJ, Oh JY, Chang HY, Yun JW, J. Biotechnol. , 127 , 129, 2006
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F, Anal. Chem. , 28 , 350, 1956
Lowry OH, Rosebrough NJ, Farr L, Randall RJ, J. Biol. Chem. , 193 , 265, 1951
Mosmann T, J. Immunol. Methods , 65 , 55, 1983
Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, Miyamae Y, Rojas E, Ryu JC, Sasaki YF, Environ. Mol. Mutagen. , 35 , 206, 2000
Selbmann L, Onofri S, Fenice M, Federici F, Petruccioli M, Res. Microbiol. , 153 , 585, 2002
Hwang HJ, Kim SW, Xu CP, Choi JW, Yun JW, J. Appl. Microbiol. , 94 (4), 708, 2003
Xu CP, Kim SW, Hwang HJ, Choi JW, Yun JW, Process Biochem. , 38 , 1025, 2002
Tang YJ, Zhong JJ, Enzyme Microb. Technol. , 32 (3-4), 478, 2003
Hayes AW, Davis ND, Diener UL, Appl. Microbiol. , 14 , 1019, 1966
Oh JY, Cho EJ, Nam SH, Choi JW, Yun JW, Process Biochem. , 42 , 3352, 2007
Hsieh C, Liu CJ, Tseng MH, Lo CT, Yang YC, Enzyme Microb. Technol. , 39 (3), 434, 2006
Baets SD, Laing SD, Francois C, Vandamme E, J. Ind. Microbiol. Biotechnol. , 29 , 181, 2002
Sobczuk TM, Camacho FG, Grima EM, Chirti Y, Bioprocess Biosyst. Eng. , 28 , 243, 2005
Godard T, Deslandes E, Lebailly P, Vigreux C, Sichel F, Poul JM, Gauduchon P, Histochem. Cell. Biol. , 112 , 155, 1999
Olive PL, Banath JP, Exp. Cell. Res. , 221 , 19, 1995
Yasuhara S, Zhu Y, Matsui T, Tipirneni N, Yasuhara Y, Kaneki M, Rosenzweig A, Martyn JA, J. Histochem. Cytochem. , 51 , 873, 2003
Yang FC, Liau CB, Process Biochem. , 33 (5), 547, 1998
Casas Lopez JL, Sanchez Perez JA, Fernandez Sevilla JM, Rodriguez Porcel EM, Chisti Y, J. Biotechnol. , 116 , 61, 2005
Moradali MF, Mostafavi H, Ghods S, Hedjaroude GA, Int. Immunopharmacol. , 7 , 701, 2007
Ooi VEC, Liu F, Int. J. Med. Mushrooms. , 11 , 195, 1999
Wang GY, Zhang J, Li H, Zhang C, Mizuno T, Ito H, Mayuzumi H, Okamoto H, Li J, Biosci. Biotechnol. Biochem. , 57 , 894, 1993
Borchers AT, Stern JS, Hackman RM, Keen CL, Gershwin ME, Proc. Soc. Exp. Biol. Med. , 221 , 281, 1999
Okazaki M, Adachi Y, Ohno N, Yadomae T, Biol. Pharm. Bull. , 18 , 1320, 1995
Shi BJ, Nie XH, Chen LZ, Liu YL, Tao WY, Carbohydr. Polymer. , 68 , 687, 2007
Lavi I, Friesem D, Geresh S, Hadar Y, Schwartz B, Cancer. Lett. , 244 , 61, 2006
Nie X, Shi B, Ding Y, Tao W, Int. J. Biol. Macromol. , 39 , 228, 2006
Wang BJ, Won SJ, Yu ZR, Su CL, Food Chem. Toxicol. , 43 , 534, 2005
Bae JS, Jang KH, Yim H, Jin HK, Cancer. Lett. , 218 , 43, 2005
Cui FJ, Li Y, Xu YY, Liu ZQ, Huang DM, Zhang ZC, Tao WY, Toxicol. In Vitro. , 21 , 417, 2007
Cao OZ, Lin ZB, Life Sci. , 78 , 1457, 2006
Lee CL, Yang XT, Wan JMF, Enzyme Microb. Technol. , 38 (1-2), 14, 2006
Li G, Kim DH, Kim TD, Park BJ, Park HD, Park JI, Na MK, Kim HC, Hong ND, Lim K, Hwang BD, Yoon WH, Cancer. Lett. , 216 , 175, 2004
Choi YH, Huh MK, Ryu CH, Choi BT, Jeong YK, Int. J. Mol. Med. , 14 , 227, 2004
Thomas RZ, Helen RG, Michael DC, Toxicology , 231 , 210, 2007
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