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Korean Journal of Chemical Engineering, Vol.23, No.4, 607-609, 2006
Purification of recombinant Pfu DNA polymerase using a new JK110 resin
The purification of recombinant Pfu DNA polymerase expressed in Escherichia coli was studied. The lysed supernatant was heated to 75 °C to denature E. coli protein, followed by chromatography on JK110 and Sephadex G-75. The purified protein had comparable activity to the commercially obtained Pfu in both DNA polymerase and PCR amplification. The final product had a specific activity of 17,600 U/mg and 149,600 U of Pfu DNA polymerase was obtained from 500ml culture. JK110 has worked well in our study and appears to be a new method of choice for purification of Pfu DNA polymerase.
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[Cited By]
- Chen HT, Lin MS, Hou SY, Korean Journal of Chemical Engineering, 25(5), 1082, 2008
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