This study innovatively proposes an integrated process of “surfactant-lipase synergistic microwave extraction and in 

situ cloud point enrichment” to address the issues of cumbersome procedures, high energy consumption, and substantial 

organic solvent usage associated with the separate steps of traditional sea buckthorn flavonoid extraction and enrichment. 

This process achieves the simultaneous and efficient completion of flavonoid extraction and concentration. It leverages the 

synergistic action of surfactant and lipase coupled with microwave assistance to rapidly disrupt sea buckthorn cell walls 

and promote flavonoid dissolution. Utilizing the cloud point characteristics of surfactants, solvent-free in situ enrichment 

is achieved through regulation of temperature and salt concentration. Under optimal conditions determined by response 

surface methodology, the yields of rutin, kaempferol, and isorhamnetin reached 1.305±0.021 mg/g, 1.724±0.018 mg/g, 

and 1.269±0.015 mg/g, respectively, representing increases of 85%, 76%, and 69% compared to the traditional reflux 

method. Scanning electron microscopy confirmed the significant cell wall disruption effect of the synergistic system. 

After optimization, the enrichment rates for rutin, kaempferol, and isorhamnetin attained 93.1±1.66%, 90.8±0.83%, and 

91.4±1.89%, respectively. This integrated process combines efficient extraction with solvent-free enrichment, offering 

operational simplicity and environmental benefits, thereby providing important technical support and theoretical reference 

for the industrial-scale green production of sea buckthorn flavonoids.