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- In relation to this article, we declare that there is no conflict of interest.
- Publication history
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Received October 24, 2024
Accepted February 25, 2025
Available online May 25, 2025
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Antioxidant-Capped Gold Nanoparticles for Colorimetric Detection of Kanamycin
https://doi.org/10.1007/s11814-025-00432-1
Abstract
In the development of aptasensors, which are biosensors that use aptamers (short DNA or RNA molecules) to specifi cally
bind to target molecules, gold nanoparticles (GNPs) have traditionally been synthesized using citric acid. However, citric
acid-capped GNPs are not optimized for constructing aptasensors. In this study, we aimed to develop a more sensitive,
selective, and effi cient antioxidant-capped GNP (A-GNP) probe for the colorimetric detection of kanamycin. We assessed
the performance of A-GNPs synthesized with polyphenols, multi-carboxylic acids, ascorbic acid, and kojic acid, as both
reducing agents and stabilizers. Among the tested antioxidants, only ascorbic acid and gallic acid mediated the formation
of A-GNPs at room temperature and these could be optimized to construct aptasensors by functionalizing the A-GNPs with
poly(adenine)-tailed DNA aptamers (pA-apt). The colorimetric probe using gallic acid-capped GNPs had a limit of detection
for kanamycin of 6.2 nM, which is lower than the 22.0 nM value obtained using citric acid-capped GNPs. Furthermore,
this aptasensor showed high selectivity for kanamycin, indicating that the A-GNP/pA-apt probe could be applied as a novel
aptasensor for antibiotic detection in real-world contexts.
