Globularia orientalis L., a member of the Oleaceae family, is well-adapted to warm climates and a medicinal and aromatic

plant traditionally used in treatment methods. In this study, 80% ethanol extract of G. orientalis was obtained, and its enzyme

inhibition activity, antioxidant, antimicrobial, and anticancer activities were investigated. Additionally, the metabolic properties

of the plant were elucidated through in silico methods. The data revealed '2-Propenoic acid, tridecyl ester' as the major

component of this plant. Furthermore, G. orientalis exhibited potent antioxidant activity, and its cytotoxic activity against

breast cancer was found to be robust. G. orientalis ' chemical constituents were shown to have diff erent actions against breast

cancer protein (PDB ID: 1A52 and 1JNX), anti-oxidant protein (PDB ID: 1HD2), AChE enzyme protein (PDB ID: 4M0E),

BChE enzyme protein (PDB ID: 5NN0), and α-Gly protein (PDB ID: 1R47). High-activity compounds were subjected to

ADME/T analysis. To investigate how compounds in the plant G. orientalis L . aff ect human metabolism, ADME/T calculations

were performed. In conclusion, these fi ndings suggest the need for continued research through activity-guided

fractionation to identify the compounds responsible for these eff ects in future studies. Lastly, the binding free energy of the

molecule with the best docking score is computed using MM/GBSA techniques.