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Language: English

Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received September 21, 2018
Accepted January 17, 2019

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Engineering Trichosporon oleaginosus for enhanced production of lipid from volatile fatty acids as carbon source

Kyungsoo Lee¹ Yong Jae Lee² Ho Nam Chang³ Ki Jun Jeong^{1 4†}

¹Department of Chemical and Biomolecular Engineering (BK21 Program), KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Korea ²Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Korea ³Lab to Market, Inc., Nurikkum Square R&D Center, 1013-2 396 World Cup buk-ro, Mapo-gu, Seoul 03925, Korea ⁴Institute for the BioCentury, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Korea

kjjeong@kaist.ac.kr

Korean Journal of Chemical Engineering, June 2019, 36(6), 903-908(6), 10.1007/s11814-018-0229-7

Abstract

Trichosporon oleaginosus is one of the most promising hosts for microbial lipid production owing to its high-productivity. In an effort to develop an economical production process, we engineered T. oleaginosus towards high-lipid production from volatile fatty acids (VFA) derived from anaerobic fermentation of food waste. First, we established a method for labeling intracellular lipid with lipophilic BODIPY fluorescent dye. Next, a random library was constructed by treatment with a chemical mutagen, and high-lipid producers were screened using fluorescenceactivated cell sorting. Subsequently, one clone, N14, was successfully isolated, which exhibited 3-fold higher lipid production (19.4%) in VFA (6 g/L) media than the wild-type strain, and also showed increased lipid production in higher concentrations of VFA (18 or 24 g/L). Based on fatty acid methyl ester (FAME) analysis, N14 contained higher stearic acid (C18:0) and oleic acid (C18:1) content compared with those of the wild-type strain.

Keywords

Trichosporon oleaginosus Volatile Fatty Acids Fluorescence-activated Cell Sorting Microbial Lipid BODIPY

References

Gujjari P, Suh SO, Coumes K, Zhou JJ, Mycologia, 103, 1110 (2011)
Yaguchi A, Rives D, Blenner M, AIMS Microbiol., 3, 227 (2017)
Meo A, Priebe XL, Weuster-Botz D, J. Biotechnol., 241, 1 (2017)
Ryu BG, Kim J, Kim K, Choi YE, Han JI, Yang JW, Bioresour. Technol., 135, 357 (2013)
Chi ZY, Zheng YB, Ma JW, Chen SL, Int. J. Hydrog. Energy, 36(16), 9542 (2011)
Xu X, Kim JY, Cho HU, Park HR, Park JM, Chem. Eng. J., 264, 735 (2014)
Gong Z, Shen H, Zhou W, Wang Y, Yang X, Zhao ZK, Biotechnol. Biofuels, 8, 189 (2015)
Kim MS, Li D, Choi OK, Sang BI, Chiang PC, Kim HO, Korean J. Chem. Eng., 34(10), 2678 (2017)
Lim SJ, Choi DW, Lee WG, Kwon S, Chang HN, Bioprocess Eng., 22, 543 (2000)
Kidanu WG, Trang PT, Yoon HH, Biotechnol. Bioprocess Eng., 22, 612 (2017)
Li D, Kim MS, Kim HJ, Choi OK, Sang BI, Chiang PC, Kim HO, Korean J. Chem. Eng., 35(1), 179 (2018)
Park GW, Fei Q, Jung K, Chang HN, Kim YC, Kim NJ, Choi JD, Kim S, Cho J, Biotechnol. J., 9, 1536 (2014)
Fei Q, Chang HN, Shang LA, Choi JDR, Kim N, Kang J, Bioresour. Technol., 102(3), 2695 (2011)
Close D, Ojumu J, Genome Announc., 4, e01235 (2016)
Kourist R, Bracharz F, Lorenzen J, Kracht ON, Chovatia M, et al., mBio, 6, e00918 (2015)
Gorner C, Redai V, Bracharz F, Schrepfer P, Garbe D, Bruck T, Green Chem., 18, 2037 (2016)
Yim SS, Bang HB, Kim YH, Lee YJ, Jeong GM, Jeong KJ, PLOS One, 9, e10822 (2014)
Choi SL, Rha E, Lee SJ, Kim H, Kwon K, Jeong YS, Rhee YH, Song JJ, Kim HS, Lee SG, ACS Synth. Biol., 3, 163 (2014)
Velmurugan N, Sung M, Yim SS, Park MS, Yang JW, Jeong KJ, Bioresour. Technol., 138, 30 (2013)
Velmurugan N, Sung M, Yim SS, Park MS, Yang JW, Jeong KJ, Biotechnol. Biofuels, 7, 117 (2014)
Yim SS, Choi JW, Lee SH, Jeong KJ, ACS Synth. Biol., 5, 334 (2014)
Hollinshead WD, Varman AM, You L, Hembree Z, Tang YJJ, Bioresour. Technol., 169, 462 (2014)
Liu J, Yuan M, Liu JN, Huang XF, Bioresour. Technol., 241, 645 (2017)
Chalima A, Oliver L, de Castro LF, Karnaouri A, Dietrich T, Topakas E, Fermentation, 3, 54 (2017)
Kameda E, Martins FF, Amaral PFF, Valoni EA, Coelho MAZ, Chem. Eng. Trans., 38, 529 (2014)
Stansell GR, Gray VM, Sym SD, J. Appl. Phycol., 24, 791 (2012)
Kinney AJ, Clemente TE, Fuel Process. Technol., 86(10), 1137 (2005)
Hoekman SK, Broch A, Robbins C, Ceniceros E, Natarajan M, Renew. Sust. Energ. Rev., 16, 143 (2012)