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Received September 27, 2016
Accepted November 20, 2016
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Identification of a new serine protease from polychaeta, Marphysa sanguinea, for its thrombolytic and anticoagulant activity
1Department of Biological Engineering, Inha University, Incheon 22212, Korea 2Department of Oceanography, Inha University, Incheon 22212, Korea 3Korea Institute of Coastal Ecology, Inc., Ojeong-gu, Bucheon 14449, Korea
hsshin@inha.ac.kr
Korean Journal of Chemical Engineering, March 2017, 34(3), 781-786(6), 10.1007/s11814-016-0331-z
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Abstract
A serine protease was purified from Marphysa sanguinea through ammonium sulfate followed by ion exchange chromatography, and its N-terminal amino sequence was identified to be IVGGSEATPYQFPFQ. Fibrinolytic activity was depended on both direct fibrinolysis and indirect plasminogen-mediated cascade and had a consistent activity irrespective of pH. The serine protease could be confirmed to degrade α-, β-, and γ-chains of human fibrinogen through fibrinogenolytic assay and did not express significant cytotoxicity to endothelial cells. These imply the enzyme has anticoagulant as well as thrombolytic activity, not significantly impairing endothelial cells comprising brain blood brain barrier (BBB) tissue. Conclusively, the new serine protease is worthy of being a candidate to substitute tissue-Plasminogen Activator (t-PA) for acute ischemic reperfusion injury of brain.
References
Libby P, Am. J. Clin. Nutr., 83, 4565 (2006)
Ingall T, J. Insur. Med., 36, 143 (2004)
Thorvaldsen P, Asplund K, Kuulasmaa K, Rajakangas AM, Schroll M, Stroke, 26, 1 (1995)
Simoons ML, Jaegere P, Domburg R, Boersma E, Maggioni AP, Franzosi MG, Leimberger JD, Califf R, Schroderm R, Knatterud G, Braunwald E, Lancet, 342, 1523 (1993)
Fromm RE, Hoskins E, Cronin L, Pratt CM, Spencer WH, Roberts R, Ann. Emerg. Med., 20, 892 (1991)
Niego B, Freeman R, Puschmann TB, Turnley AM, Medcalf RL, Blood, 119, 4752 (2012)
Eisenberg PR, Sherman LA, Tiefenbrunn AJ, Ludbrook PA, Sobel BE, Jaffe AS, Thromb. Haemost., 57, 35 (1987)
Nilsson T, Wallen P, Mellbring G, Eur. J. Haematol., 33, 49 (1984)
Tsikouris JP, Tsikouris AP, Pharmacotherapy, 21, 207 (2001)
Matsuo O, Rijken DC, Collen D, Thromb. Haemost., 45, 225 (1981)
Hacke W, Albers G, Ai-Rawi Y, Bogousslavsky J, Davalos A, Eliasziw M, Fischer M, Furlan A, Kaste M, Lees KR, Soehgen M, Warach S, Stroke, 36, 1 (2005)
Albers GW, von Kummor R, Truelsen T, Jensen JS, Ravn GM, Grønning BA, Chabriat H, Chang K, Davalos AE, Ford GA, Grotta J, Kaste M, Schwamm LH, Shuaib A, Lancet. Neurol., 14, 575 (2015)
Loscalzo J, Braunwald E, N. Engl. J. Med., 319, 925 (1988)
Wiman B, Collen D, Nature, 272, 549 (1978)
Matys T, Strickland S, Nat. Med., 9, 371 (2003)
Micieli G, Marcheselli S, Tosi PA, Vasc. Health Risk Manag., 5, 397 (2009)
Su EJ, Fredriksson L, Geyer M, Folestad E, Cale J, Andrae J, Gao Y, Pietras K, Mann K, Yepes M, Strickland DK, Betsholtz C, Eriksson U, Lawrence DA, Nat. Med., 14, 731 (2008)
Liu D, Cheng T, Guo H, Fernandez JA, Griffin JH, Song X, Zlokovic BV, Nat. Med., 10, 1379 (2004)
Benchenane K, Lopez-Atalaya JP, Fernandez-Monreal M, Touzani O, Vivien D, Trends Neurosci., 27, 155 (2004)
Kaur J, Zhao Z, Klein GM, Lo EH, Buchan AM, J. Cereb. Blood. Flow. Metab., 24, 945 (2004)
Deng ZH, Wang SH, Li Q, Ji X, Zhang LZ, Hong M, Bioresour. Technol., 101(6), 1954 (2010)
Zhang Y, Cui J, Zhang R, Wang Y, Hong M, Biochimie, 89, 93 (2007)
Matsubara K, Hori K, Matsuura Y, Miyazawa K, Comp. Biochem. Physiol. B, Biochem. Mol. Biol., 125, 137 (2000)
Wang S, Deng Z, Li Q, Ge X, Bo Q, Liu J, Cui J, Jiang X, Liu J, Zhang L, Hong M, Comp. Biochem. Physiol. B, Biochem. Mol. Biol., 159, 18 (2011)
Collen D, Thromb. Haemost., 82, 259 (1999)
Matsuo O, Rijken DC, Collen D, Nature, 291, 590 (1981)
Camiolo SM, Thorsen S, Astrup T, Exp. Biol. Med., 138, 227 (1971)
Kotb E, Appl. Biochem. Microbiol., 51, 34 (2015)
James GT, Anal. Biochem., 86, 574 (1978)
Choi JH, Sapkota K, Kim S, Kim SJ, Biochimie, 105, 45 (2014)
Camiolo SM, Thorsen S, Astrup T, Exp. Biol. Med., 138, 277 (1971)
Isaev NK, Stelmashook EV, Plotnikov EY, Khryapenkova TG, Lozier ER, Doludin YV, Silachev DN, Zorov DB, Biochem., 73, 1171 (2008)
Alexandrov AV, Grotta JC, Neurology, 59, 862 (2002)
Pizzo SV, Schwartz ML, Hill RL, Mckee PA, J. Clin. Invest., 51, 2841 (1972)
Farrell DH, Thiagarajan P, Chung DW, Davie EW, Proc. Natl. Acad. Sci. U. S. A., 89, 10729 (1992)
Ouyang C, Huang TF, Biochim. Biophys. Acta - Enzymology, 571, 270 (1979)
Lee JW, Seu JH, Rhee IK, Jin I, Kawamura Y, Park W, Biochem. Biophys. Res. Commun., 260, 665 (1999)
Park JW, Park JE, Choi HK, Jung TW, Yoon SM, Lee JS, Process Biochem., 48(5-6), 979 (2013)
Ingall T, J. Insur. Med., 36, 143 (2004)
Thorvaldsen P, Asplund K, Kuulasmaa K, Rajakangas AM, Schroll M, Stroke, 26, 1 (1995)
Simoons ML, Jaegere P, Domburg R, Boersma E, Maggioni AP, Franzosi MG, Leimberger JD, Califf R, Schroderm R, Knatterud G, Braunwald E, Lancet, 342, 1523 (1993)
Fromm RE, Hoskins E, Cronin L, Pratt CM, Spencer WH, Roberts R, Ann. Emerg. Med., 20, 892 (1991)
Niego B, Freeman R, Puschmann TB, Turnley AM, Medcalf RL, Blood, 119, 4752 (2012)
Eisenberg PR, Sherman LA, Tiefenbrunn AJ, Ludbrook PA, Sobel BE, Jaffe AS, Thromb. Haemost., 57, 35 (1987)
Nilsson T, Wallen P, Mellbring G, Eur. J. Haematol., 33, 49 (1984)
Tsikouris JP, Tsikouris AP, Pharmacotherapy, 21, 207 (2001)
Matsuo O, Rijken DC, Collen D, Thromb. Haemost., 45, 225 (1981)
Hacke W, Albers G, Ai-Rawi Y, Bogousslavsky J, Davalos A, Eliasziw M, Fischer M, Furlan A, Kaste M, Lees KR, Soehgen M, Warach S, Stroke, 36, 1 (2005)
Albers GW, von Kummor R, Truelsen T, Jensen JS, Ravn GM, Grønning BA, Chabriat H, Chang K, Davalos AE, Ford GA, Grotta J, Kaste M, Schwamm LH, Shuaib A, Lancet. Neurol., 14, 575 (2015)
Loscalzo J, Braunwald E, N. Engl. J. Med., 319, 925 (1988)
Wiman B, Collen D, Nature, 272, 549 (1978)
Matys T, Strickland S, Nat. Med., 9, 371 (2003)
Micieli G, Marcheselli S, Tosi PA, Vasc. Health Risk Manag., 5, 397 (2009)
Su EJ, Fredriksson L, Geyer M, Folestad E, Cale J, Andrae J, Gao Y, Pietras K, Mann K, Yepes M, Strickland DK, Betsholtz C, Eriksson U, Lawrence DA, Nat. Med., 14, 731 (2008)
Liu D, Cheng T, Guo H, Fernandez JA, Griffin JH, Song X, Zlokovic BV, Nat. Med., 10, 1379 (2004)
Benchenane K, Lopez-Atalaya JP, Fernandez-Monreal M, Touzani O, Vivien D, Trends Neurosci., 27, 155 (2004)
Kaur J, Zhao Z, Klein GM, Lo EH, Buchan AM, J. Cereb. Blood. Flow. Metab., 24, 945 (2004)
Deng ZH, Wang SH, Li Q, Ji X, Zhang LZ, Hong M, Bioresour. Technol., 101(6), 1954 (2010)
Zhang Y, Cui J, Zhang R, Wang Y, Hong M, Biochimie, 89, 93 (2007)
Matsubara K, Hori K, Matsuura Y, Miyazawa K, Comp. Biochem. Physiol. B, Biochem. Mol. Biol., 125, 137 (2000)
Wang S, Deng Z, Li Q, Ge X, Bo Q, Liu J, Cui J, Jiang X, Liu J, Zhang L, Hong M, Comp. Biochem. Physiol. B, Biochem. Mol. Biol., 159, 18 (2011)
Collen D, Thromb. Haemost., 82, 259 (1999)
Matsuo O, Rijken DC, Collen D, Nature, 291, 590 (1981)
Camiolo SM, Thorsen S, Astrup T, Exp. Biol. Med., 138, 227 (1971)
Kotb E, Appl. Biochem. Microbiol., 51, 34 (2015)
James GT, Anal. Biochem., 86, 574 (1978)
Choi JH, Sapkota K, Kim S, Kim SJ, Biochimie, 105, 45 (2014)
Camiolo SM, Thorsen S, Astrup T, Exp. Biol. Med., 138, 277 (1971)
Isaev NK, Stelmashook EV, Plotnikov EY, Khryapenkova TG, Lozier ER, Doludin YV, Silachev DN, Zorov DB, Biochem., 73, 1171 (2008)
Alexandrov AV, Grotta JC, Neurology, 59, 862 (2002)
Pizzo SV, Schwartz ML, Hill RL, Mckee PA, J. Clin. Invest., 51, 2841 (1972)
Farrell DH, Thiagarajan P, Chung DW, Davie EW, Proc. Natl. Acad. Sci. U. S. A., 89, 10729 (1992)
Ouyang C, Huang TF, Biochim. Biophys. Acta - Enzymology, 571, 270 (1979)
Lee JW, Seu JH, Rhee IK, Jin I, Kawamura Y, Park W, Biochem. Biophys. Res. Commun., 260, 665 (1999)
Park JW, Park JE, Choi HK, Jung TW, Yoon SM, Lee JS, Process Biochem., 48(5-6), 979 (2013)
