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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received December 26, 2013
Accepted June 12, 2014

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Enhanced production of carboxymethylcellulase of Bacillus subtilis subsp. subtilis A-53 by a recombinant Escherichia coli JM109/A-53 with pH and temperature shifts

Eun-Jung Lee Wa Gao¹ Jin-Woo Lee^1†

Department of Medical Bioscience, Graduate School of Dong-A University, Busan 604-714, Korea ¹Department of Biotechnology, Dong-A University, Busan 604-714, Korea

jwlee@dau.ac.kr

Korean Journal of Chemical Engineering, January 2015, 32(1), 113-117(5), 10.1007/s11814-014-0160-x

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Abstract

Effect of shifts in pH of the medium and temperature on cell growth and production of carboxymethylcellulase (CMCase) by a recombinant Escherichia coli JM109/A-53 was investigated. Lower initial pH (pH 7.0) of the medium and higher temperature (40 ℃) stimulated cell growth of E. coli JM109/A-53, whereas higher initial pH (pH 8.0) and lower temperature (35 ℃) enhanced production of CMCase. The maximal production of CMCase was obtained when initial pH of 7.0 and temperature of 40 ℃ were simultaneously changed to 8.0 and 35 ℃ after 36 h. The production of CMCase with simple shifts in pH and temperature was 783.1 U/mL, which was 2.13 times higher than that under optimal conditions for cell growth. This indicated that a simple process with shifts in pH and temperature could be economically applied for enhanced production of CMCase by E. coli JM109/A-53 with industrial scales.

Keywords

Carboxymethylcellulase Escherichia coli JM109 Optimization Shifts in pH and Temperature

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