Articles & Issues

Language: English

Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received July 14, 2012
Accepted February 11, 2013

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

All issues

Utilization of cane molasses for docosahexaenoic acid production by Schizochytrium sp. CCTCC M209059

Lu-Jing Ren Juan Li Yuan-Wei Hu Xiao-Jun Ji He Huang^†

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing 210009, P. R. China

biotech@njut.edu.cn

Korean Journal of Chemical Engineering, April 2013, 30(4), 787-789(3)
https://doi.org/10.1007/s11814-013-0020-0

Download PDF

Abstract

Cane molasses (CM), an agro-industrial by-product, was first examined for docosahexaenoic acid (DHA) production by Schizochytrium sp. Cell dry weight as 21.94 g/L at treated CM cultivation was similar to that at pure glucose (26.7 g/L) cultivation. Batch fermentation at different initial CM concentration showed that DHA percentage could reach 47.51% at 10 g/L CM but only 37.90% at 70 g/L CM. By analyzing the fermentation process, monosodium glutamate might be a positive agent for effective DHA production. Finally, monosodium glutamate and malic acid were_x000D_ introduced to the fed-batch fermentation for effective DHA production.

Keywords

Docosahexaenoic Acid Schizochytrium sp. Cane Molasses Lipid Monosodium Glutamate

References

Agostoni C, Riva E, Trojan S, Bellu R, Giovannini M, Lancet., 346, 638 (1995)
Sijtsma L, de Swaaf ME, Appl. Microbiol. Biotechnol., 64(2), 146 (2004)
Ganuza E, Anderson AJ, Ratledge C, Biotechnol. Lett., 30(9), 1559 (2008)
Liang YN, Sarkany N, Cui Y, Blackburn JW, Bioresour. Technol., 101(17), 6745 (2010)
Unagal P, Assantachai C, Phadungruengluij S, Suphantharika M, Tanticharoen M, Verduyn C, Bioresour. Technol., 98(2), 281 (2007)
Liang Y, Sarkany N, Cui Y, Yesuf J, Trushenski J, Blackburn JW, Bioresour. Technol., 101, 3023 (2010)
Liu YP, Zheng P, Sun ZH, Ni Y, Dong JJ, Zhu LL, Bioresour. Technol., 99, 1736 (2010)
El-Enshasy HA, Mohamed NA, Farid MA, El-Diwany AI, Bioresour. Technol., 99(10), 4263 (2008)
Li N, Deng ZN, Qin YL, Chen CL, Liang ZQ, Food Technol. Biotechnol., 46, 73 (2008)
Ren LJ, Huang H, Xiao AH, Lian M, Ji XJ, Bioproc. Biosyst. Eng., 32, 837 (2009)
Ye Q, Li XM, Yan M, Cao H, Xu L, Zhang YY, Chen Y, Xiong J, Ouyang PK, Ying HJ, Appl. Microbiol. Biotechnol., 87(2), 517 (2010)
Miller GL, Anal. Chem., 31, 426 (1959)
Ren LJ, Ji XJ, Huang H, Qu LA, Feng Y, Tong QQ, Ouyang PK, Appl. Microbiol. Biotechnol., 87(5), 1649 (2010)
Yu LJ, Qin WM, Lan WZ, Zhou PP, Zhu M, Bioresour. Technol., 88(3), 265 (2003)
Ratledge C, Biochimie., 86, 807 (2004)
Edmund M, Wise J, Eric GB, Proc. Natl. Acad Sci., 52(5), 1255 (1964)
Wedding RT, Plant Physiol., 90, 367 (1989)
Lan WZ, Qin WM, Yu LJ, Lett. Appl. Microbiol., 35, 357 (2002)