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Received November 15, 2010
Accepted January 16, 2011
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Determination of optimal conditions for ribonucleic acid production by Candida tropicalis no. 121
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, P. R. China
Korean Journal of Chemical Engineering, August 2011, 28(8),
10.1007/s11814-011-0013-9
10.1007/s11814-011-0013-9
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Abstract
The experiments were based on multivariate statistical concepts, and response surface methodology (RSM) was applied to optimize the fermentation medium for the production of ribonucleic acid (RNA) by Candida tropicalis no. 121. The process involved the individual adjustment and optimization of various medium components at shake flask level. The two-level Plackett-Burman (PB) design was used to screen the medium components, which significantly_x000D_
influenced RNA production. Among seven variables, the concentrations of molasses, ZnSO4, and H3PO4 were found to be the important factors that significantly affected RNA production (confidence levels above 95%). These factors were further optimized using a central composite design (CCD) and RSM. The optimum values for the critical components were as follows: molasses 47.21 g/L: ZnSO4 0.048 g/L; H3PO4 1.19 g/L. Under optimal conditions, RNA production was 2.56 g/L, which was in excellent agreement with the predicted value (2.561 g/L), and led to a 2.1-fold increase compare with that using the original medium in RNA production.
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References
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