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Received January 19, 2010
Accepted March 2, 2010
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Two-step bioprocess employing whole cell and enzyme for economical biodiesel production
Department of Environmental and Applied Chemical Engineering, Gangneung-Wonju National University, Gangneung, Gangwon-do 210-702, Korea
Korean Journal of Chemical Engineering, September 2010, 27(5), 1555-1559(5), 10.1007/s11814-010-0263-y
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Abstract
A preliminary research for feasible and economical bioprocess for biodiesel production was carried out. A microorganism producing lipase was isolated from grease-contaminated soil. After chemically mutating twice, the microorganism exhibited 2.5 times higher intracellular lipase activities than wild type one and was used as a whole cell biocatalyst for biodiesel production. 0.1% toluene treatment followed by freeze drying was the optimum preparation method for whole cell biocatalyst. 1 g whole cell biocatalyst corresponded to 0.15 g commercial enzyme under mild methanol inhibition condition, while corresponding to 0.7 g enzyme under severe methanol inhibition environment. A two-step process, biodiesel production by the whole cell and sequentially by commercial enzyme, was suggested. This novel process, combining the advantages of whole cell and enzyme, saved the usage of commercial enzyme and alleviated enzyme deactivation by methanol.
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Li L, Du W, Liu D, Wang L, Li Z, J. Molecular Catal. B: Enzymatic., 43, 58 (2006)
Ban K, Kaieda M, Matsumoto T, Kondo A, Fukuda H, Biochem. Eng., 8, 39 (2001)
Hama S, Yamaji H, Fukumizu T, Numata T, Tamalampudi S, Kondo A, Nodac H, Fukuda H, Biochem. Eng. J., 34, 273 (2007)
Tamalampudi S, Talukder MR, Hama S, Numata T, Kondo A, Fukuda H, Biochem. Eng. J., 39(1), 185 (2008)
Li W, Du W, Liu D, Process Biochem., 42, 1481 (2007)
Matsumoto T, Takahashi S, Kaieda M, Ueda M, Tanaka A, Fukuda H, Kondo A, Appl. Microbiol. Biotechnol., 57(4), 515 (2001)
Du W, Li W, Sun T, Chen X, Liu DH, Appl. Microbiol. Biotechnol., 79(3), 331 (2008)
Jaeger KE, Ransac S, Dijkstra BW, Colson C, Vanheuvel M, Misset O, Fems Microbiol. Rev., 15, 29 (1994)
Lee DW, Koh YS, Kim KJ, Kim BC, Choi HJ, Kim DS, Suhartono MT, Ryun YR, FEMS Micobiol. Lett., 179, 393 (1999)
Hung TC, Giridgar R, Chiou SH, Wu WT, J. Mol. Catal.B: Enzym., 26, 69 (2003)
Schwab AW, Bagby MO, Freedman B, Fuel., 66, 1372 (1987)
Chae JC, Kim CK, Gerben JZ, Biochem. Biophy. Res. Commun., 338, 1600 (2005)
Yu L, Xu Y, Wang X, Yu X, J. Molecular Catal. B: Enzymatic., 47, 149 (2007)
Flores MV, Voget CE, Ertola RJ, J. Chem. Technol. Biotechnol., 64(4), 353 (1995)
Jeon DJ, Yeom SH, Bioprocess Biosys. Eng., Submitted (2010)
