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Received September 8, 2009
Accepted October 27, 2009
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Overexpression of ethionine resistance gene for maximized production of S-adenosylmethionine in Saccharomyces cerevisiae sake kyokai No. 6
Department of Chemical & Biological Engineering, Korea University, 5-1 Anam-dong, Sungbuk-gu, Seoul 136-713, Korea 1Samyang Genex, 63-2 Hwaam-dong, Yuseong-gu, Daejeon 305-717, Korea
mkoh@korea.ac.kr
Korean Journal of Chemical Engineering, February 2010, 27(2), 587-589(3), 10.1007/s11814-010-0100-3
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Abstract
In our previous work, we developed an auxotrophic mutant of Saccharomyces cerevisiae sake kyokai No. 6 strain for the selection of recombinant DNA. Sake yeast can accumulate high amount of S-adenosylmethionine (SAM) that is effective for treating depression, Alzheimer’s disease and so on. In this work, we further introduced SAM2 gene and ERC1, a frame-shift mutant of YHR032w, to maximize the production of S-adenosylmethionine in S. cerevisiae sake K6. The recombinant strain accumulated SAM about half of its dry cell weight.
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Mato JM, Corrales FJ, Lu SC, Avila MA, Faseb J., 16, 15 (2002)
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Shiomi N, Fukuda H, Fukuda Y, Murata K, Kimura A, J. Ferment. Bioeng., 71, 211 (1991)
Shobayashi M, Ukena E, Fujii T, Iefuji H, Biosci. Biotechnol. Biochem., 71, 323 (2007)
Budovskaya YV, Stephan JS, Deminoff SJ, Herman PK, Proc. Natl. Acad. Sci. USA, 102, 13933 (2005)
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Roje S, Wang H, McNeil SD, Raymond RK, Appling DR, Shachar-Hill Y, Bohnert HJ, Hanson AD, J. Biol. Chem., 274, 36089 (1999)
