Articles & Issues
- Language
- English
- Conflict of Interest
- In relation to this article, we declare that there is no conflict of interest.
- Publication history
-
Received August 25, 2008
Accepted October 26, 2008
-
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright © KIChE. All rights reserved.
All issues
Improvement of sensitivity in an interferometry by controlling pore size on the anodic aluminum oxide chip pore-widening technique
Department of Chemical Engineering, Sungkyunkwan University, Suwon 440-746, Korea
wkim@skku.ac.kr
Korean Journal of Chemical Engineering, January 2009, 26(1), 160-164(5), 10.1007/s11814-009-0026-9
Download PDF
Abstract
The pore size of an anodic aluminum oxide (AAO) chip, as well as uniform pore distribution, is one of the key parameters that should be adjusted, by choosing the appropriate etching conditions, in order to enhance the sensitivity of an interferometer. In this study, the pore size of AAO chips was optimized and characterized in order to lower the detection limit of prostate specific antigen (PSA) in an interferometric immunoassay system. After pore widening for 30-50 min, the AAO pore size was increased approximately 2-fold larger than that before pore widening. A large increase in effective optical density (ΔEOT) was obtained from the AAO chip fabricated by pore-widening technique, which thereby lowered the PSA detection limit. The present study results are not sufficiently validated to enable the immediate application to immunoassay for prostate cancer (PCa) screening, but they do demonstrate that controlling_x000D_
pore size can positively affect the sensitivity and lower the detection limit.
Keywords
References
Schwartz MP, Alvarez SD, Sailor MJ, Anal. Chem., 79, 327 (2007)
Gao T, Rothberg LJ, Anal. Chem., 79, 7589 (2007)
Janshoff A, Dancil KPS, Steinem C, Greiner DP, Lin VSY, Gurtner C, Motesharei K, Sailor MJ, Ghadiri MR, J. Am. Chem. Soc., 120(46), 12108 (1998)
Lee JC, An JY, Kim BW, J. Chem. Technol. Biotechnol., 82(11), 1045 (2007)
Park JS, Lim SH, Sim SJ, Chae H, Yoon HC, Yang SS, Kim BW, J. Microbiol. Biotechnol., 16, 1968 (2006)
Lee W, Nielsch K, Gosele U, Nanotechnology, 18, 475713 (2007)
Ghorbani M, Nasirpouri F, Irajizad A, Saedi A, Mater. Des., 27, 983 (2006)
Ji GB, Chen W, Tang SL, Gu BX, Li Z, Du YW, Solid State Commun., 130, 541 (2004)
Lee I, Wool RP, Thin Solid Films, 379(1-2), 94 (2000)
Metzger RM, Konovalov VV, Sun M, Xu T, Zangari G, Xu B, Benakli M, Doyle WD, IEEE Trans. Magn., 36, 30 (2000)
Mizeikisa V, Juodkazis S, Marcinkevicius A, Matsuo S, Misawa H, J. Photochem. Photobiol. C: Photochem. Rev., 2, 35 (2001)
Sui YC, Saniger JM, Mater. Lett., 48, 127 (2001)
Huang L, Reekmans G, Saerens D, Friedt JM, Frederix F, Francis L, Muyldermans S, Campitelli A, Van Hoof C, Biosens. Bioelectron., 21, 483 (2005)
Cao C, Kim JP, Kim BW, Chae H, Yoon HC, Yang SS, Sim SJ, Biosens. Bioelectron., 21, 2106 (2006)
Wesseling S, Stephan C, Semjonow A, Lein M, Brux B, Sinha P, Loering SA, Jung K, Clin. Chem., 49, 887 (2003)
Fernadez-Sanchez C, McNeil CJ, Rawson K, Nilsson O, Anal. Chem., 76, 5649 (2004)
Balk SP, Ko YJ, Burbley GJ, J. Clin. Oncol., 21, 383 (2003)
Healy DA, Hayes CJ, Leonard P, McKenna L, O’Kennedy R, Trends Biotechnol., 25, 125 (2007)
Oberpenning F, Hetzel S, Weining C, Brandt B, Angelis GA, Heinecke A, Lein M, Fornara P, Schmid H, Hertle L, Semjonow A, Eur. Urol., 43, 478 (2003)
Masuda H, Fukuda K, Science, 268(5216), 1466 (1995)
Hwang SK, Jeong SH, Hwang HY, Lee OJ, Lee KH, Korean J. Chem. Eng., 19(3), 467 (2002)
Gao T, Rothberg LJ, Anal. Chem., 79, 7589 (2007)
Janshoff A, Dancil KPS, Steinem C, Greiner DP, Lin VSY, Gurtner C, Motesharei K, Sailor MJ, Ghadiri MR, J. Am. Chem. Soc., 120(46), 12108 (1998)
Lee JC, An JY, Kim BW, J. Chem. Technol. Biotechnol., 82(11), 1045 (2007)
Park JS, Lim SH, Sim SJ, Chae H, Yoon HC, Yang SS, Kim BW, J. Microbiol. Biotechnol., 16, 1968 (2006)
Lee W, Nielsch K, Gosele U, Nanotechnology, 18, 475713 (2007)
Ghorbani M, Nasirpouri F, Irajizad A, Saedi A, Mater. Des., 27, 983 (2006)
Ji GB, Chen W, Tang SL, Gu BX, Li Z, Du YW, Solid State Commun., 130, 541 (2004)
Lee I, Wool RP, Thin Solid Films, 379(1-2), 94 (2000)
Metzger RM, Konovalov VV, Sun M, Xu T, Zangari G, Xu B, Benakli M, Doyle WD, IEEE Trans. Magn., 36, 30 (2000)
Mizeikisa V, Juodkazis S, Marcinkevicius A, Matsuo S, Misawa H, J. Photochem. Photobiol. C: Photochem. Rev., 2, 35 (2001)
Sui YC, Saniger JM, Mater. Lett., 48, 127 (2001)
Huang L, Reekmans G, Saerens D, Friedt JM, Frederix F, Francis L, Muyldermans S, Campitelli A, Van Hoof C, Biosens. Bioelectron., 21, 483 (2005)
Cao C, Kim JP, Kim BW, Chae H, Yoon HC, Yang SS, Sim SJ, Biosens. Bioelectron., 21, 2106 (2006)
Wesseling S, Stephan C, Semjonow A, Lein M, Brux B, Sinha P, Loering SA, Jung K, Clin. Chem., 49, 887 (2003)
Fernadez-Sanchez C, McNeil CJ, Rawson K, Nilsson O, Anal. Chem., 76, 5649 (2004)
Balk SP, Ko YJ, Burbley GJ, J. Clin. Oncol., 21, 383 (2003)
Healy DA, Hayes CJ, Leonard P, McKenna L, O’Kennedy R, Trends Biotechnol., 25, 125 (2007)
Oberpenning F, Hetzel S, Weining C, Brandt B, Angelis GA, Heinecke A, Lein M, Fornara P, Schmid H, Hertle L, Semjonow A, Eur. Urol., 43, 478 (2003)
Masuda H, Fukuda K, Science, 268(5216), 1466 (1995)
Hwang SK, Jeong SH, Hwang HY, Lee OJ, Lee KH, Korean J. Chem. Eng., 19(3), 467 (2002)
