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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received January 18, 2003
Accepted February 19, 2003

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Mobile Phase Composition for Resolving Whey Proteins in Reversed-Phase High Performance Liquid Chromatography

Yong An Jung Du Young Choi Seung Bum Hong Kyung Ho Row^†

Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University, 253 Yonghyun-dong, Nam-ku, Incheon 402-751, Korea

rowkho@inha.ac.kr

Korean Journal of Chemical Engineering, July 2003, 20(4), 705-708(4)
https://doi.org/10.1007/BF02706912

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Abstract

Reversed-phase high-performance liquid chromatography was successfully developed for the simultaneous and rapid separation for the main whey proteins, α-Lactalbumin and β-Lactoglobulin. This method consisted of a linear gradient of the two mobile phases of 0.1% trifluoroacetic acid in water and 0.1% trifluoroacetic acid in acetonitrile. The total run time for this separation was approximately 30 min, and α-Lactalbumin was eluted followed by β-Lactoglobulin. The injection volume was fixed at 20 μl and the flow rate was 1 ml 1/min. The optimum mobile phase composition and gradient conditions to separate α-Lactalbumin and β-Lactoglobulin (A+B) were experimentally obtained at the 15 μm particle with a pore size of 300 Å on the linear-gradient mode.

Keywords

Column Liquid Chromatography Reversed-Phase Separation Whey Proteins α-Lactalbumin β-Lactoglobulin A and B

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