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Received January 22, 2002
Accepted March 10, 2002
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Chromatographic Analysis of Ceramide III of Saccharomyces cerevisiae
Mi Soon Yeo1
Duk Hui Kang1
Se Kyung Kim1 2
Hyun Shik Yun1
Yoon Mo Koo1
Youn-Woo Lee3
Kyung Ho Row1 2†
1Center for Advanced Bioseparation Technology, Inha University, 253 Yonghyun-Dong, Nam-Ku, Incheon 402-751, Korea 2Div. of Chem. Eng., Inha University, 253 Yonghyun-Dong, Nam-Ku, Incheon 402-751, Korea 3National Research Lab. for Supercritical Fluid, KIST, Seoul 136-791, Korea
rowkho@inha.ac.kr
Korean Journal of Chemical Engineering, September 2002, 19(5), 815-817(3)
https://doi.org/10.1007/BF02706973
https://doi.org/10.1007/BF02706973
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Abstract
Ceramide was prepared by the cultivation of Saccharomyces cerevisiae from cell extracts by solvent extraction and analyzed by NP-HPLC using a UV detector. The mobile phase was composed of hexane, methanol, and IPA. From the experimental conditions, the composition of mobile phase was 72/5/23 (hexane/IPA/methanol, vol%). Quantitative analysis of ceramide was performed. Based on the analytical conditions, the effect of cultivation temperature for the production of ceramide was investigated and the optimum cultivation temperature was found to be 35 ℃.
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Zhou JY, Chaminade P, Gaudin K, Prognon P, Baillet A, Ferrier D, J. Chromatogr. A, 859, 99 (1999)
