Graphene draws considerable attention among biomedical researchers because of its unique physical, chemical and biological properties. The wide applications of graphene in the biomedical arena such as diagnostics, drug immobilization and drug delivery were well documented in the literature. However the therapeutic potential of the graphene towards retroviruses and the interactions of the graphene with receptors/proteins are still unexplored. Herein we report the antagonistic molecular interactions of graphene with the three key target proteins of HIV infections namely HIVVpr, Nef and Gag proteins. The docking investigations were performed to find the binding energy of the graphene ligands to the key target proteins of HIV. The high binding affinity of the graphene to these proteins indicates the antagonistic molecular interaction of graphene to the disease targets. The therapeutic potential of graphene was also studied by changing the size and the number of layers of the graphene. The experimental results confirm the good therapeutic potential of the graphene to combat HIV mediated retroviral nfections.