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HWAHAK KONGHAK,
Vol.37, No.2, 330-335, 1999
Vol.37, No.2, 330-335, 1999
특성화된 poly(hydroxyethyl-methacrylate) 비드를 이용한 human immunoglobulin G의 흡착분리에 관한 연구(I) - Thiophilic poly(hydroxyethyl-methacryate) 비드의 제조 -
A Study on Adsorptive Separation of Human Immunoglobulin G with the Characterized Poly (hydroxyethyl-methacrylate) Beads (I) - Preparation of Thiophilic Poly (hydroxyethyl-methacrylate) Beads -
Divinylsulfone(DVS)과 β-mercaptoethanol을 이용한 일련의 반응을 통해, 흡착담체 조건에 맞게 제조된 poly(hydroxyethyl-methacrylate)(PHEMA) 비드를 thiophilic-특성화하고자 하였다. 실험변수들은 Na2CO3 농도, pH, 반응시간 그리고 각 반응물의 농도로 하였으며, 반응물의 결합량이 최대값을 얻을 때 변수를 고정하였다. DVS를 통한 PHEMA의 활성화 반응에 있어서, PHEMA비드들의 가교현상없이 얻을 수 있는 최대 결합랑은 Na2CO3 0.5 mol/L, pH 12.5, 반응 시간 8 시간 그리고 DVS 2 mol/L에서 약 16.2mmol DVS/g PHEMA이었다. 또한 활성화된 PHEMA(APHEMA)와 β-mercaptoethanol을 반응시킨 결합반응에서는 Na2CO3 0.25 mol/L, pH 9, 반응 시간 36시간 그리고 β-mercaptoethanol 4 mol/L에서 최대값인 약 514.4 μmol β-mercaptoethanol/g APHEMA를 얻었다.
PoIy(hydroxyethyl-methacrylate)(PHEMA) beads, prepared in accordance with the standards of adsorptive matrix, were tried to have the thiophilic characters by a series of reactions with divinylsulfone(DVS) and -mercaptoethanol. The experiments parameters were Na2CO3 concentration, pH, reaction time and the concentration of the reactants. When the bound mass was maximum, the parameter fixed. In the activation step of PHEMA with DVS, the maximum bound quantity of DVS was 16.2mmol DVS/g PHEMA at Na2CO3 0.5 mol/L pH 12.5, reaction time 8hr and DVS 2mol/L. And in the coupling reaction with the activated PHEMA(APHEMA) and -mercaptoethanol, the fixed parameters were Na2CO3 0.25 mol/L, pH 9, reaction time 36 hr and -mercaptoethanol 4 mol/L for the upmost mass of about 514.4 mol -mercaptoethanol/g APHEMA.
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