| Issue |
Korean Chemical Engineering Research,
Vol.50, No.3, 545-550, 2012
Vol.50, No.3, 545-550, 2012
반응 표면 분석법을 사용한 새우껍질에서 astaxanthin 추출 조건의 최적화
Optimization Condition of Astaxanthin Extract from Shrimp Waste Using Response Surface Methodology
최적의 추출조건을 찾는데 매우 유용한 방법인 반응 표면 분석법(RSM, response surface methodology)을 사용하여 새우껍질로부터 astaxanthin 추출조건을 최적화하였다. 추출조건은 용매 에탄올과 추출물질의 비율, 추출온도(℃), 추출시간(min)의 세가지 독립변수를 설정하여 BBD (Box-Behnken design) 방법을 이용하였다. 이 BBD 모델링은 0.9218의 R2adj값과 0.0003의 확률 값 p 값으로 회귀 모델에 대한 신뢰도를 입증하였다. RSM 분석을 통해 찾아낸 새우껍질로부터 astaxanthin의 최적 추출조건은 에탄올 용매비 1:29.7, 추출온도 49.5 ℃, 추출시간 59.9 분이고, 이 때 astaxanthin 추출량은 17.80 μg/g으로 예측하였다. 최적 수율로 예측된 결과는 각각의 조건에 따른 실험을 통해 그 예측의 정확도를 확인하였으며 17.77 μg/g으로 예측조건과 비슷한 결과를 보였다.
A 17-run Box-Behnken design (BBD) was used to optimize the extraction conditions of astaxanthin from shrimp waste. Three factors such as ratio of ethanol to raw material, extraction temperature (℃) and extraction time (min) were investigated. The adjusted coefficient of determination (R2adj) for the model was 0.9218, and the probability value (p=0.0003) demonstrated a high significance for the regression model. The optimum extraction conditions were found to be: optimized ratio of ethanol to raw material 29.7, extraction temperature 49.5 ℃ and extraction time 59.9 min. Under these conditions, the mean extraction yield of astaxanthin was 17.80 μg/g, which was in good agreement with the predicted model value. Under these conditions, validation experiments were done and the mean extraction yield of astaxanthin was 17.77 μg/g, which is in good agreement with the predicted model value.
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- Park SM, Ko KY, Kim IH, Korean Chemical Engineering Research, 53(6), 717, 2015
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