(+)-Troger’s base in Troger’s base racemates that inhibits thromboxane A2(T×A2) synthase has been used to treat arteriosclerosis. Separation of (+)-Troger’s base by chromatography has become a major concern. However separation experiments of (+)-Troger’s base need time and consumables so that simulation with Aspen Chromatography could save time and costs by predicting the efficiency of separation. Injection amount and eluent flow rate were varied to compare the resolutions and yields of TB(-) and TB(+). Highest resolution and yield were attained at the eluent rate of 0.25 mL/min. Isotherms representing the relationship between mobile phase concentration and stationary phase concentration were changed to get the best separation with Ideal Adsorbed Solution(IAS) Statistical Lanmuir isotherms.