| Issue |
Korean Chemical Engineering Research,
Vol.44, No.2, 166-171, 2006
Vol.44, No.2, 166-171, 2006
압전 재료의 탄성표면파 특성과 단백질의 고정화
Surface Acoustic Wave Characteristics of Piezoelectric Materials and Protein Immobilization
본 연구에서는 전기적 결합 계수가 큰 PMN-PT 압전 재료를 사용하여 탄성표면파를 발진시켜 단백질을 검출할 수있는 새로운 바이오센서로써 이용 가능성을 확인하고자 시도하였다. 실험결과 PMN-PT 압전 재료의 중심 주파수 필터링은 LT 압전 재료보다 우수하였지만, 만족할만한 결과를 얻을 수는 없었다. 또한, 본 연구에서는 위암을 일으키는mismatched DNA를 검출하기 위한 방법을 개발하고자 하였다. 그 결과 EDC 용액을 사용하여 NTA에 MutS를 고정화하였다. 그러나 Ni(니켈)을 사용하여 MutS를 고정화하여 mismatched DNA를 측정하는 것이 더 효과적인 방법이라 판단된다.
In this study, in using a piezoelectric material of Pb(Mg1/3Nb2/3)O3-PbTiO3 (PMN-PT), which has a highelectromechanical coupling coefficient, we have tried to study about this material can be practically available as a newbiosensor to detect protein by using surface acoustic wave (SAW). As the results, the filtering of the center frequency ofthe PMN-PT substrate is a superior result to that of the LiTaO3 (LT) substrate, but the result was not completely satis-factory. Also this study attempts to develop a sensing method to detect mismatched DNA in order to diagnose cancer.We could directly immobilize the MutS to the NTA using the EDC solution. But, we immobilized MutS using nickel andit is judged that is more effective method to detect mismatched DNA.
Keywords:
Surface Acoustic Wave Biosensor; Electro-mechanical Coupling Coefficient; PMN-PT; Protein Immobilization
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