| Issue |
Korean Chemical Engineering Research,
Vol.43, No.3, 419-424, 2005
Vol.43, No.3, 419-424, 2005
차가버섯의 균사체 성장에 대한 배지성분의 영향
Effect of Medium Compositions on the Mycelial Growth of Inonotus obliquus
차가버섯(Inonotus obliquus) 균사체의 액체배양에서 온도(22-32 ℃)와 pH(5-7) 그리고 배지성분의 변화가 차가버섯 균사체의 성장에 미치는 연구를 수행하였다. Glucose, starch, peptone, yeast extract, K2HPO4, MgSO4·7H2O 그리고 CaCl2를 각각 30-120 g/L, 0-10 g/L, 0-20 g/L, 0-15 g/L, 0-2 g/L, 0-1.5 g/L, 0-0.5 g/L의 범위로 변화시키면서 실험하였다. 차가버섯 균사체의 성장속도는 26-27 ℃, pH 6, glucose 70 g/L, yeast extract 5 g/L 그리고 CaCl2 0.1 g/L일 때에 최대값을 나타났다. Starch와 peptone 그리고 K2HPO4의 농도가 증가할수록 차가버섯 균사체의 성장속도는 증가하였으나, MgSO4·7H2O의 농도가 증가할수록 차가버섯 균사체의 성장속도는 감소하였다. 차가버섯 균사체의 최대 성장 속도를 위한 액체배지는 glucose 70 g/L, peptone 5-20 g/L, starch 10 g/L, yeast extract 5 g/L, K2HPO4 2 g/L 그리고 CaCl2 0.1 g/L로 구성된다.
. Effect of temperature(22-32 ℃), pH(5-7) and medium composition on the mycelial growth for the submerged culture of Inonotus obliquus. The concentrations of glucose, starch, peptone, yeast extract, K2HPO4, MgSO4· 7H2O and CaCl2 were examined in the ranges of 30-120 g/L, 0-10 g/L, 0-20 g/L, 0-15 g/L, 0-2 g/L, 0-1.5 g/L and 0-0.5g/L, respectively. The maximum mycelial growth of Inonotus obliquus was obtained for 26-27 ℃ and pH 6. The concentrations of glucose, yeast extract and CaCl2, which gave the maximum mycelial growth of Inonotus obliquus, were 70 g/L, 5 g/L and 0.1 g/L, respectively. In the cases of starch, peptone and K2HPO4, the mycelial growth of Inonotus obliquus increased with increasing the concentrations. However, as the concentration of MgSO4·7H2O increased, the mycelial growth of Inonotus obliquus decreased. The medium for maximum mycelial growth of Inonotus obliquus consisted of (per 1 L): glucose, 70 g; peptone, 5-20 g; starch, 10 g; yeast extract, 5 g; K2HPO4, 2 g and CaCl2, 0.1 g.
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